era

era
168

GTP-binding protein, involved in the assembly of the 30S ribosomal subunit

Locus
BSU_25290
Molecular weight
33.92 kDa
Isoelectric point
8.38
Protein length
301 aaSequenceBlast
Gene length
906 bpSequenceBlast
Function
ribosome assembly
Product
GTP-binding protein
Essential
yes
Synonyms
era, bex, yqfH
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1159 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,610,041 → 2,610,946
Phenotypes of a mutant
essential PubMed
The protein
Catalyzed reaction/ biological activity
Binds and hydrolyzes GTP and readily exchanges GDP for GTP
Protein family
TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily (according to UniProt)
Domains
Era-type G domain (aa 7-174) (according to UniProt)
KH type-2 domain (aa 205-282) (according to UniProt)
Structure
3IEV (PDB) (Era from Aquifex aeolicus, 50% identity, 77% similarity, complex with the 3' end of 16S rRNA) PubMed
P42182 (AlphaFold)
Effectors of protein activity
binds ppGpp PubMed, ppGpp may inhibit activity PubMed
Paralogous protein(s)
YsxC, RbgA, ThdF, Obg, YqeH, YnbA, EngA, YyaF
Localization
cytoplasm, throughout the cell PubMed
Expression and Regulation
Operons
Genes
cdd-era-yqzL-recO
Description
PubMed
Expression
expression is increased upon depletion of depletion of tRNA maturation factors rnpB or rnz PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigB: sigma factor, PubMed, in sigB regulon
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cdd→recO

2025-04-06 03:24:21

Jstuelk

93

F5B4B68A066EAD6341E5A2A464B577EB9CEAEDD4

CB199ECFB0BAFE3DBDF1FFE046AD4EA2F93B5339

Biological materials
Expression vectors
for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP837, available in Stülke lab
for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP845, available in Stülke lab
Labs working on this gene/protein
Naotake Ogasawara, Nara, Japan
References
Reviews
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Original Publications
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CB68B3393CA7862C45C624AF7B1ADD188243A01F

Page visits: 4850

Time of last update: 2025-04-08 20:24:03

Author of last update: Christoph.elfmann