Difference between revisions of "DesK"

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* '''Description:''' two-component sensor kinase, regulation of  cold shock expression of des <br/><br/>
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* '''Description:''' [[two-component systems|two-component]] sensor kinase, regulation of  cold shock expression of ''[[des]]'' <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || two-component sensor kinase (NarL family)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || [[two-component systems|two-component]] sensor kinase  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of  cold shock expression of des
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|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of  cold shock expression of ''[[des]]''
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU19190 desK]
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=DesK DesK]
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=desK desK]'''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 9.428   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 9.428   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[des]]'', ''[[desR]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[des]]'', ''[[desR]]''
 
|-
 
|-
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13811&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU19190 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU19190 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU19190 DNA_with_flanks]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yocF_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yocF_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 +
|-
 +
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=desK_2090574_2091686_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:desK_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU19190]]
 
|-
 
|-
 
|}
 
|}
  
 
__TOC__
 
__TOC__
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/>
 +
 +
= [[Categories]] containing this gene/protein =
 +
{{SubtiWiki category|[[lipid metabolism/ other]]}},
 +
{{SubtiWiki category|[[protein modification]]}},
 +
{{SubtiWiki category|[[transcription factors and their control]]}},
 +
{{SubtiWiki category|[[cold stress proteins]]}},
 +
{{SubtiWiki category|[[membrane proteins]]}},
 +
{{SubtiWiki category|[[phosphoproteins]]}}
 +
 +
= This gene is a member of the following [[regulons]] =
  
−
<br/><br/>
 
  
 
=The gene=
 
=The gene=
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU19190&redirect=T BSU19190]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yocFG.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yocFG.html]
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=== Additional information===
 
=== Additional information===
 +
 +
  
  
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
−
* '''Catalyzed reaction/ biological activity:''' ATP + protein L-histidine = ADP + protein N-phospho-L-histidine (according to Swiss-Prot)
+
* '''Catalyzed reaction/ biological activity:''' autophosphorylation, phosphorylation of [[DesR]]
−
 
+
* '''Protein family:'''  
−
* '''Protein family:''' peptidase S66 family (according to Swiss-Prot)
 
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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* '''Domains:'''  
 
* '''Domains:'''  
 +
** 5 transmembrane helices
 +
** cytoplasmatic C-terminal trail
  
−
* '''Modification:'''
+
* '''Modification:''' autophosphorylation on a His residue
  
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
  
−
* '''Effectors of protein activity:'''
+
* '''Effectors of protein activity:''' unsaturated fatty acids are negative effectors of the system
  
−
* '''Interactions:'''
+
* '''[[SubtInteract|Interactions]]:'''
 +
** [[DesK]]-[[DesR]]
  
−
* '''Localization:'''
+
* '''[[Localization]]:''' membrane (transmembrane segments)
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU19190&redirect=T BSU19190]
  
−
* '''Structure:'''
+
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3EHF 3EHF]
  
−
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O34757 O34757]
+
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34757 O34757]
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU19190]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU19190]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
  
 
=== Additional information===
 
=== Additional information===
 +
*[[DesK]] has the ability to sense changes in membrane fluidity {{PubMed|17087771}}
  
 
=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:'''  
+
* '''Operon:''' ''[[desK]]-[[desR]]'' {{PubMed|11285232}}
  
−
* '''Sigma factor:'''  
+
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=desK_2090574_2091686_1 desK] {{PubMed|22383849}}
 +
 
 +
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** induced by cold shock (12-fold) {{PubMed|12399512}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
−
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
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=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
−
 
+
* [[Diego de Mendoza]], Universidad Nacional de Rosario, Argentine [http://www.ibr.gov.ar/eng/investigacion/demendoza.htm homepage]
−
[[Mohamed Marahiel]], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]
+
* [[Mohamed Marahiel]], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]
  
 
=Your additional remarks=
 
=Your additional remarks=
  
 
=References=
 
=References=
 +
==Reviews==
 +
<pubmed> 20117042, 17087771 24819366 </pubmed>
  
−
<pubmed>10094672,14734164,12399512,, 19233289 </pubmed>
+
==Original publications==
−
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
+
<pubmed>10094672,14734164,12399512,20507988 , 19233289 11285232 19805278 15090506, 12207704 20705470 23356219 24574048 24522108 25406381 26172072 </pubmed>
 +
[[Category:Protein-coding genes]]

Latest revision as of 09:21, 15 July 2015

  • Description: two-component sensor kinase, regulation of cold shock expression of des

Gene name desK
Synonyms yocF
Essential no
Product two-component sensor kinase
Function regulation of cold shock expression of des
Gene expression levels in SubtiExpress: desK
Interactions involving this protein in SubtInteract: DesK
Metabolic function and regulation of this protein in SubtiPathways:
desK
MW, pI 42 kDa, 9.428
Gene length, protein length 1110 bp, 370 aa
Immediate neighbours des, desR
Sequences Protein DNA DNA_with_flanks
Genetic context
YocF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DesK expression.png















Categories containing this gene/protein

lipid metabolism/ other, protein modification, transcription factors and their control, cold stress proteins, membrane proteins, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU19190

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of DesR
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • 5 transmembrane helices
    • cytoplasmatic C-terminal trail
  • Modification: autophosphorylation on a His residue
  • Cofactor(s):
  • Effectors of protein activity: unsaturated fatty acids are negative effectors of the system

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

  • DesK has the ability to sense changes in membrane fluidity PubMed

Expression and regulation

  • Regulation:
    • induced by cold shock (12-fold) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews


Original publications

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