dacC

dacC
168

Class C penicillin-binding protein 4A, D-alanyl-D-alanine carboxypeptidase

Locus
BSU_18350
Molecular weight
52.72 kDa
Isoelectric point
5.41
Protein length
Gene length
Function
carboxypeptidase
Product
penicillin-binding protein 4A, D-alanyl-D-alanine carboxypeptidase
Essential
no
Synonyms
dacC, pbp

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2027 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,998,340  1,999,815
The protein
Catalyzed reaction/ biological activity
cleavage ofD-Ala-D-Ala interpeptide bridges in peptidoglycan PubMed
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine (according to UniProt)
Protein family
peptidase S13 family (single member, according to UniProt)
Structure
2J9P (PDB) (complex with a peptidoglycan mimetic peptide) PubMed
during vegetative growth: distinct foci and bands at cell periphery PubMed
secreted, may be anchored in the membrane via a C-terminal amphiphatic alpha helix PubMed
Expression and Regulation
Operons
Genes
Description
Sigma factors
SigH: sigma factor, PubMed, in sigH regulon
SigB: sigma factor, PubMed, in sigB regulon
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galMdacC

2025-02-16 03:41:24

ghost

66

84afbdb41d180106aa15bcff435af91360f0b0dc

F8A98803534B1962350B5A88E0E1D29F0924DB0C

Biological materials
Mutant
MGNA-B075 (dacC::erm), available at the NBRP B. subtilis, Japan
BKE18350 (dacC::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTAATTTCCCCGCCTCTC,  downstream forward: _UP4_TAATAGCGGGAGACCTGTTT
BKK18350 (dacC::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTAATTTCCCCGCCTCTC,  downstream forward: _UP4_TAATAGCGGGAGACCTGTTT
GFP fusion
3102 trpC2 (amyE::Pxyl-gfp-dacC1-1473 spc) PubMed, available in Dirk Jan Scheffers' lab and in the BGSC
3104 trpC2 dacC::pSG5044 (cat Pxyl-gfpa-dacC1-615) PubMed, available in Dirk Jan Scheffers' lab and in the BGSC
References
Reviews
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Original Publications
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20A3120D2A45449745C9162EDCF386ED3163851B

Page visits: 3265

Time of last update: 2025-02-23 09:01:02

Author of last update: Jstuelk