icd

icd
168

isocitrate dehydrogenase

Locus
BSU_29130
Molecular weight
46.26 kDa
Isoelectric point
4.83
Protein length
423 aaSequenceBlast
Gene length
1272 bpSequenceBlast
Function
TCA cycle
Product
isocitrate dehydrogenase
Essential
no
E.C.
1.1.1.42
Synonyms
icd, citC
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0538 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,979,716 2,980,987
Phenotypes of a mutant
reduced ability to sporulate PubMed
growth and sporulation defects of the mutant could be partially bypassed by deletion of the major citrate synthase gene (''citZ'') PubMed
The protein
Catalyzed reaction/ biological activity
isocitrate + NADP+ --> 2-oxoglutarate + CO2 + NADPH (according to UniProt)
Protein family
Isocitrate and isopropylmalate dehydrogenases family (with LeuB and YcsA, according to UniProt)
Cofactors
Mg2+, Mn2+, NADP+
Structure
1HQS (PDB) PubMed
P39126 (AlphaFold)
Modification
phosphorylated on Arg-180 PubMed
phosphorylated PubMed, PubMed
in vitro phosphorylated by PrkC on Thr-138, Thr-147, and Thr-396 PubMed
Effectors of protein activity
Inhibited by glyoxylate, oxaloacetate and oxalomalate PubMed
Paralogous protein(s)
YcsA
Additional information
This enzyme requires NADP+ exclusively. No activity was seen on the presence on NAD+ PubMed
extensive information on the structure and enzymatic properties of Icd can be found at Proteopedia
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
icd-mdh
Description
PubMed
Regulation
''citZ'': catabolite repression (CcpA) PubMed
Regulatory mechanism
CcpA: repression, CcpC: transcription repression PubMed, in ccpA regulon
CcpC: repression, (molecular inducer: citrate) PubMed, in ccpC regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

icd→mdh

2025-04-20 11:04:15

ghost

120

f9ad3d456cafa7c9074216bd662b61b54e3995fc

2BD9E142645FECA776F7762A7431A0FB5A5D9070

Genes
citZ-icd-mdh
Description
PubMed
Regulation
''CitZ'': catabolite repression (CcpA) PubMed
Regulatory mechanism
CcpA: repression, CcpC: transcription repression PubMed, in ccpA regulon
CcpC: repression, (molecular inducer: citrate) PubMed, in ccpC regulon
CitB: mRNA stability control, upon citrate accumulation or iron limitation PubMed, in citB regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

citZ→mdh

2025-04-08 16:35:19

Jstuelk

133

d65e97dee2d196244ee89dd42bbf730b7ea9c870

F01A9D3586F0AD300B6C4F086E56F8AEA52D457F

Biological materials
Mutant
GP666 (spc), GP672 (erm), available in Jörg Stülke's lab
1A1000 ( icd::spec), PubMed, available at BGSC
1A999 ( icd::spec), PubMed, available at BGSC
GP790 (''citZ-icd-mdh)::kan'', available in Jörg Stülke's lab
GP2331 (''citZ-icd-mdh)::kan, Cre-recombinase is integrated in sacA'',available in Jörg Stülke's lab
GP2333 (''citZ-icd-mdh)::lox72, Cre-recombinase is integrated in sacA'', available in Jörg Stülke's lab
BKE29130 (icd::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAATAAAAACCTCCCAGT, downstream forward: _UP4_TAAGCAAGGAAAAAGCCTAA
BKK29130 (icd::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAATAAAAACCTCCCAGT, downstream forward: _UP4_TAAGCAAGGAAAAAGCCTAA
Expression vectors
pGP1121 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab) PubMed
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
Antibody
available in Linc Sonenshein's lab
Labs working on this gene/protein
Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
References
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CB7D0E32C044D541CD966DC1F9DA489D518A7703

Page visits: 7759

Time of last update: 2025-04-20 21:02:07

Author of last update: Jstuelk