trpB

trpB
168

tryptophan synthase (beta subunit)

Locus
BSU_22640
Molecular weight
43.42 kDa
Isoelectric point
5.53
Protein length
400 aaSequenceBlast
Gene length
1200 bpSequenceBlast
Function
biosynthesis of tryptophan
Product
tryptophan synthase (beta subunit)
Essential
no
E.C.
4.2.1.20
Synonyms
trpB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0133 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,372,304 → 2,373,506
The protein
Catalyzed reaction/ biological activity
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine --> D-glyceraldehyde 3-phosphate + H2O + L-tryptophan (according to UniProt)
Protein family
TrpB family (single member, according to UniProt)
Cofactors
PLP (according to UniProt)
Structure
4NEG (PDB) (from Bacillus anthracis, 60% identity)
P07600 (AlphaFold)
Expression and Regulation
Operons
Genes
trpE-trpD-trpC-trpF-trpB-trpA
Description
PubMed
Regulation
not expressed if tryptophan is available (MtrB) PubMed
the mRNA is substantially stabilized upon depletion of RNase Y PubMed
the RNA switch RNA is degraded by RNase Y PubMed
Regulatory mechanism
MtrB: transcription termination, PubMed, in mtrB regulon
Open in new tab

trpE→trpA

2025-03-26 09:36:59

Jstuelk

87

53eef6e8739e58d1bed57b78e584c86a2e6ff379

37D11B58C2266E362D5C193ADEA68D53F64A90E3

Genes
trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE
Description
PubMed
Regulation
not expressed if tryptophan is available (MtrB) PubMed
the mRNA is substantially stabilized upon depletion of RNase Y PubMed
Regulatory mechanism
MtrB: transcription termination, PubMed, in mtrB regulon
Open in new tab

trpE→aroE

2025-04-05 00:44:39

Jstuelk

117

a3b37416002f0f1e65847bd8d15ba18b53400752

AA88BDB7192FA5467301E36C102EEEBB21BEDA86

Biological materials
Mutant
BKE22640 (ΔtrpB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCCAAAATCACCGTATCTGC,  downstream forward: _UP4_GTATTGGAAGAAGAGGTGAA
BKK22640 (ΔtrpB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCCAAAATCACCGTATCTGC,  downstream forward: _UP4_GTATTGGAAGAAGAGGTGAA
References
Reviews
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Original Publications
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AEB1F4E28C5B2AFC7FCF65721D4D1A70D6FC38EE

Page visits: 3486

Time of last update: 2025-04-09 11:13:23

Author of last update: Jstuelk