fliW

fliW
168

checkpoint protein for hag expression, CsrA antagonist

Locus
BSU_35380
Molecular weight
16.03 kDa
Isoelectric point
4.34
Protein length
Gene length
Function
control of CsrA activity
Product
flagellar assembly factor, CsrA antagonist
Essential
no
Synonyms
fliW, yviF

Genomic Context

List of homologs in different organisms, belongs to COG1699 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, SigD regulon, ComK regulon, DegU regulon, ScoC regulon

Gene
Coordinates
3,636,264  3,636,695
The protein
Catalyzed reaction/ biological activity
FliW binds CsrA when Hag is secreted after hook completion (and FliW released from the Hag-FliW complex) to allow translation of the'' hag'' mRNA PubMed
Protein family
fliW family (single member, according to UniProt)
Structure
5DMD (PDB) (from Geobacillus thermodenitrificans) PubMed
cytoplasm (according to Swiss-Prot)
Additional information
CsrA and FliW form a conserved module in many bacteria PubMed
Expression and Regulation
Operons
Description
Expression
expression is heterogeneous PubMed
Regulatory mechanism
ComK: activation, PubMed, in comK regulon
DegU: activation, DegU-P, PubMed, in degU regulon
ScoC: repression, PubMed, in scoC regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigD: sigma factor, PubMed, in sigD regulon
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comFAcsrA

2025-01-08 02:13:49

Jstuelk

145

F13A7084BCCB15345BC26C03AF15EE753EFDF196

11180672E46D33F7D747E58441ECB10AEE7F8D8B

Biological materials
Mutant
MGNA-A339 (yviF::erm), available at the NBRP B. subtilis, Japan
BKE35380 (fliW::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTGTTCACGATCCTTTTC,  downstream forward: _UP4_AAGCATCCGATTGGAGGAGA
BKK35380 (fliW::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTGTTCACGATCCTTTTC,  downstream forward: _UP4_AAGCATCCGATTGGAGGAGA
References
Reviews
Mukherjee S, Kearns DB The structure and regulation of flagella in Bacillus subtilis. Annual review of genetics. 2014; 48:319-40. doi:10.1146/annurev-genet-120213-092406. PMID:25251856
Romeo T, Vakulskas CA, Babitzke P Post-transcriptional regulation on a global scale: form and function of Csr/Rsm systems. Environmental microbiology. 2013 Feb; 15(2):313-24. doi:10.1111/j.1462-2920.2012.02794.x. PMID:22672726
Original Publications
O'Reilly FJ, Graziadei A, Forbrig C, Bremenkamp R, Charles K, Lenz S, Elfmann C, Fischer L, Stülke J, Rappsilber JProtein complexes in cells by AI-assisted structural proteomics.Molecular systems biology. 2023 Feb 23; :e11544. PMID: 36815589
Oshiro RT, Dunn CM, Kearns DBContact with the CsrA core is required for allosteric inhibition by FliW in Bacillus subtilis.Journal of bacteriology. 2020 Oct 26; . PMID: 33106347
Oshiro RT, Rajendren S, Hundley HA, Kearns DB Robust Stoichiometry of FliW-CsrA Governs Flagellin Homeostasis and Cytoplasmic Organization in Bacillus subtilis. mBio. 2019 May 21; 10(3). pii:e00533-19. doi:10.1128/mBio.00533-19. PMID:31113895
Altegoer F, Rensing SA, Bange G Structural basis for the CsrA-dependent modulation of translation initiation by an ancient regulatory protein. Proceedings of the National Academy of Sciences of the United States of America. 2016 Sep 06; 113(36):10168-73. doi:10.1073/pnas.1602425113. PMID:27551070
Mukherjee S, Oshiro RT, Yakhnin H, Babitzke P, Kearns DB FliW antagonizes CsrA RNA binding by a noncompetitive allosteric mechanism. Proceedings of the National Academy of Sciences of the United States of America. 2016 Aug 30; 113(35):9870-5. doi:10.1073/pnas.1602455113. PMID:27516547
Arrieta-Ortiz ML, Hafemeister C, Bate AR, Chu T, Greenfield A, Shuster B, Barry SN, Gallitto M, Liu B, Kacmarczyk T, Santoriello F, Chen J, Rodrigues CD, Sato T, Rudner DZ, Driks A, Bonneau R, Eichenberger P An experimentally supported model of the Bacillus subtilis global transcriptional regulatory network. Molecular systems biology. 2015 Nov 17; 11(11):839. doi:10.15252/msb.20156236. PMID:26577401
Mukherjee S, Babitzke P, Kearns DB FliW and FliS function independently to control cytoplasmic flagellin levels in Bacillus subtilis. Journal of bacteriology. 2013 Jan; 195(2):297-306. doi:10.1128/JB.01654-12. PMID:23144244
Mukherjee S, Yakhnin H, Kysela D, Sokoloski J, Babitzke P, Kearns DB CsrA-FliW interaction governs flagellin homeostasis and a checkpoint on flagellar morphogenesis in Bacillus subtilis. Molecular microbiology. 2011 Oct; 82(2):447-61. doi:10.1111/j.1365-2958.2011.07822.x. PMID:21895793
Titz B, Rajagopala SV, Ester C, Häuser R, Uetz P Novel conserved assembly factor of the bacterial flagellum. Journal of bacteriology. 2006 Nov; 188(21):7700-6. . PMID:16936039

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Time of last update: 2025-01-17 01:34:35

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