amiE

amiE
168

N-acetylmuramyl-L-alanine amidase

Locus
BSU_01670
Molecular weight
49.14 kDa
Isoelectric point
9.35
Protein length
441 aaSequenceBlast
Gene length
1326 bpSequenceBlast
Function
recycling of cell wall material
Product
N-acetylmuramyl-L-alanine amidase
Essential
no
Synonyms
amiE, ybbE
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1680 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
188,408 → 189,733
The protein
Catalyzed reaction/ biological activity
hydrolyzes the amide bond between MurNAc and the L-alanine residue of the stem peptide PubMed
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
Protein family
peptidase S12 family (single member, according to UniProt)
Structure
4IVK (PDB) (from uncultured bacterium, 25% identity) PubMed
O05213 (AlphaFold)
Expression and Regulation
Operons
Genes
murQ-murR-murP-amiE-nagZ-namZ
Description
PubMed
Regulation
expressed in late exponential and early stationary phase PubMed
Regulatory mechanism
MurR: repression, PubMed, in murR regulon
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murQ→namZ

2025-04-03 15:04:28

Jstuelk

150

63a61d46d8ccef554859655721937254bb90adc4

0CB715DED62C09573A88E4102ECFE473836124B4

Biological materials
Mutant
BKE01670 (ΔamiE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGGCTAGCCCCTTTCTG,  downstream forward: _UP4_TGAATGATTAGACGATAGGA
BKK01670 (ΔamiE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGGCTAGCCCCTTTCTG,  downstream forward: _UP4_TGAATGATTAGACGATAGGA
References
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Time of last update: 2025-04-15 05:22:58

Author of last update: Melvin.boenninger