cysK

cysK
168

cysteine synthase, trigger enzyme, acts as corepressor for CymR

Locus
BSU_00730
Molecular weight
32.67 kDa
Isoelectric point
5.49
Protein length
308 aaSequenceBlast
Gene length
927 bpSequenceBlast
Function
biosynthesis of cysteine, control of CymR activity
Product
cysteine synthase
Essential
no
E.C.
2.5.1.47
Synonyms
cysK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0031 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
81,771 82,697
Phenotypes of a mutant
constitutive expression of the genes of the CymR regulon
a mccA cysK double mutant is auxotrophic for cysteine PubMed
The protein
Catalyzed reaction/ biological activity
O(3)-acetyl-L-serine + H2S --> L-cysteine + acetate (according to UniProt)
Protein family
Cysteine synthase/cystathionine beta-synthase family (with MccA and YtkP, according to UniProt)
Cofactors
PLP (according to Swiss-Prot)
Structure
2EGU (PDB) (from Geobacillus kaustophilis, 84% identity)
1Y7L (PDB) (from Haemophilus influenzae, 39% identity, 52% similarity) PubMed
P37887 (AlphaFold)
Paralogous protein(s)
MccA, YtkP
Localization
cytoplasm (according to UniProt)
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
coaX-yacC-yacD-cysK
Description
PubMed
Regulation
induced by heat stress (Spx) PubMed
Regulatory mechanism
Spx: activation, PubMed, in spx regulon
Sigma factors
SigM: sigma factor, PubMed, in sigM regulon
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coaX→cysK

2025-04-08 17:42:23

Jstuelk

143

9ef1e909d5470f714eecab6c2a37a360ff5a937a

003644EA8BBEA39BF437B882590C41426A16D68B

Genes
cysK
Description
PubMed
Regulation
repressed by casamino acids PubMed
Regulatory mechanism
CymR: repression, PubMed, in cymR regulon
Spx: activation, PubMed, in spx regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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cysK

2025-03-01 21:38:04

ghost

101

39f1c124b5522da23540e3707675b8dfdac1d535

3D7A683C64E1C94E02DED689C80BD7981FC39ADF

Biological materials
Mutant
GP3386 (trpC2 ΔcysK::neo), available in Jörg Stülke's lab PubMed
GP4462 (trpC2 ΔcysK::lox72), available in Jörg Stülke's lab PubMed
1A834 ( cysK::kan), PubMed, available at BGSC
1A801 ( cysK::spec), PubMed, available at BGSC
1A803 ( cysK::spec), PubMed, available at BGSC
1A946 ( cysK::spec), PubMed, available at BGSC
BKE00730 (cysK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTCGACACCTCAATTT, downstream forward: _UP4_TAAAAAAAGCCAAAACTCCC
BKK00730 (cysK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTCGACACCTCAATTT, downstream forward: _UP4_TAAAAAAAGCCAAAACTCCC
Labs working on this gene/protein
[[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France
References
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D1FC976597E5583E40A4ED7234FBCA743AB01354

Page visits: 7300

Time of last update: 2025-04-15 22:22:33

Author of last update: Jstuelk