purF

purF
168

glutamine phosphoribosyldiphosphate amidotransferase

Locus
BSU_06490
Molecular weight
51.45 kDa
Isoelectric point
5.87
Protein length
Gene length
Function
purine biosynthesis
Product
glutamine phosphoribosyldiphosphate amidotransferase
Essential
no
E.C.
2.4.2.14
Synonyms
purF, purB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0034 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
705,441  706,871
The protein
Catalyzed reaction/ biological activity
5-phospho-β-D-ribosylamine + diphosphate + L-glutamate --> 5-phospho-α-D-ribose 1-diphosphate + H2O + L-glutamine (according to UniProt)
Protein family
Glutamine amidotransferase type-2 domain (aa 12-231) (according to UniProt)
Fe-S cluster PubMed
Structure
1GPH (PDB)1AO0 (PDB) (complex with ADP and GMP)
Additional information
subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and Regulation
Operons
Description
Regulation
expression activated by glucose (4.4 fold) PubMed
Regulatory mechanism
G-box: RNA switch, (riboswitch) PubMed, in G-box
PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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purEpurD

2025-01-23 20:30:19

Jstuelk

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ace69dc80f4f087486c218727fa370a7a9630d21

EDD8EC37B0E5B38941D5827C265AB2F23CF23EA7

Biological materials
Mutant
BKE06490 (purF::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CCCAAAAACGCCGCATTCTT,  downstream forward: _UP4_TAAAACTTGAAAAATGACAT
BKK06490 (purF::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CCCAAAAACGCCGCATTCTT,  downstream forward: _UP4_TAAAACTTGAAAAATGACAT
References
Reviews
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Original Publications
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4AA5AF9C7CE8D0A9FD3A47650ECF130BCBEE1952

Page visits: 4814

Time of last update: 2025-01-24 08:17:21

Author of last update: Melvin.boenninger