nth

nth
168

apurinic/apyrimidinic endonuclease , endonuclease III, removes abasic sites during base excision repair

Locus
BSU_22340
Molecular weight
24.85 kDa
Isoelectric point
9.28
Protein length
219 aaSequenceBlast
Gene length
660 bpSequenceBlast
Function
DNA repair
Product
endonuclease III
Essential
no
Synonyms
nth, jooB, ypoB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0177 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,344,755 → 2,345,414
Phenotypes of a mutant
the mutant is sensititive to exposure to H2O2 PubMed
The protein
Catalyzed reaction/ biological activity
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate (according to UniProt)
Protein family
Nth/MutY family (with MutY, according to UniProt)
Domains
HhH domain (aa 109-128) (according to UniProt)
Cofactors
Fe-S cluster PubMed
Structure
1P59 (PDB) (complex with DNA, Geobacillus stearothermophilus)
P39788 (AlphaFold)
Effectors of protein activity
activity is stimulated by untwisting of the DNA (by DnaD, Hbs, or YonN) PubMed
Expression and Regulation
Operons
Genes
asnS-dnaD-nth-ypoC
Description
PubMed
Open in new tab

asnS→ypoC

2025-04-03 07:01:27

Jstuelk

131

bddd14432a7dcf7c9a63e653f673da9509b2f9e3

2131D7452E334EEE4F3058582EAA4C1674387033

Genes
dnaD-nth
Description
PubMed
Regulation
constitutively expressed PubMed
Open in new tab

dnaD→nth

2025-04-03 06:57:59

ghost

113

65d592657688b69134681e5f3b8625d248b380d2

2611FA0607D22ABE843DF3A9F08C088FA9A52577

Biological materials
Mutant
BKE22340 (Δnth::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAACACGATTGTCACCTTTT,  downstream forward: _UP4_GATAAAAAAGGACTGGTGAA
BKK22340 (Δnth::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAACACGATTGTCACCTTTT,  downstream forward: _UP4_GATAAAAAAGGACTGGTGAA
References
Reviews
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Original Publications
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C352560155F5B2E951B5059794D7B319C1A3AF2C

Page visits: 1848

Time of last update: 2025-04-07 09:12:27

Author of last update: Melvin.boenninger