iolE

iolE
168

2-keto-myo-inositol dehydratase, dehydration of 2-keto-myo-inositol (2nd reaction)

Locus
BSU_39720
Molecular weight
33.44 kDa
Isoelectric point
5.21
Protein length
Gene length
Function
myo-inositol catabolism
Product
2-keto-myo-inositol dehydratase, dehydration of 2-keto-myo-inositol (2nd reaction)
Essential
no
E.C.
4.2.1.44
Synonyms
iolE, yxdE

Genomic Context

List of homologs in different organisms, belongs to COG1082 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CcpA regulon, IolR regulon

Gene
Coordinates
4,078,173 → 4,079,066
The protein
Catalyzed reaction/ biological activity
scyllo-inosose --> 3D-3,5/4-trihydroxycyclohexane-1,2-dione + H2O (according to UniProt)
Protein family
IolE/MocC family (single member, according to UniProt)
Structure
3CNY (PDB) (from Lactobacillus plantarum wcfs1 mutant, 63% identity, 78% similarity)
Expression and Regulation
Operons
Description
Regulation
induced by inositol (IolR) PubMed
Regulatory mechanism
IolR: repression, PubMed, in iolR regulon
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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iolAiolJ

2025-01-11 21:13:47

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4fd092965338ef1511600132442d2247a2693c98

C19E63046F0B412961D2626F72E0308D922D50B2

Biological materials
Mutant
MGNA-B774 (iolE::erm), available at the NBRP B. subtilis, Japan
BKE39720 (ΔiolE::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_TTTGCCCATCTATGGTGCCT,  downstream forward: _UP4_CTGGCTTAATGGGGAATGAA
BKK39720 (ΔiolE::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_TTTGCCCATCTATGGTGCCT,  downstream forward: _UP4_CTGGCTTAATGGGGAATGAA
Labs working on this gene/protein
Yasutaro Fujita, University of Fukuyama, Japan
[[Ken-ichi Yoshida]], Kobe University, Japan
References
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Page visits: 4819

Time of last update: 2025-01-18 04:01:14

Author of last update: Melvin.boenninger