alr

alr
168

alanine racemase

Locus
BSU_04640
Molecular weight
43.10 kDa
Isoelectric point
8.43
Protein length
389 aaSequenceBlast
Gene length
1167 bpSequenceBlast
Function
peptidoglycan biosynthesis
Product
alanine racemase
Essential
yes
E.C.
5.1.1.1
Synonyms
alr, dal, alrA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0787 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
517,372 → 518,541
Phenotypes of a mutant
reported to be essential PubMed, however deletion mutants have been reported by Ferrari et al. (1985) and Xia et al (2007)  PubMed
auxotrophy for D-alanine
a alaP alr double mutant is not viable due to the inability to acquire D-alanine PubMed
the mutant is not viable in liquid culture but it can live in a mixed biofilm (with wild type) due to D-ala sharing PubMed
The protein
Catalyzed reaction/ biological activity
L-alanine --> D-alanine (according to UniProt)
Protein family
alanine racemase family (with YncD, according to UniProt)
Structure
8ZPE (PDB) PubMed
1L6G (PDB) (with N-[5'-phosphopyridoxyl]-D-alanine, Geobacillus stearothermophilus)
1SFT (PDB) (Geobacillus stearothermophilus)
P10725 (AlphaFold)
Paralogous protein(s)
YncD
Expression and Regulation
Operons
Genes
alr-ndoAI-ndoA
Description
PubMed
Open in new tab

alr→ndoA

2025-04-04 09:47:10

Jstuelk

89

91b7fa89916159e2bc7894e263572f1eeb4a5554

F1ED0F7D4D8E54352AA236431B9C138B1FC5ADBA

Biological materials
Mutant
alr-1, two-base mutation 526AG>T (=R176X), creating a frameshift and an immediate stop codon, available in BGSC 1A4, a D-alanine auxotroph
References
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Time of last update: 2025-04-15 17:33:09

Author of last update: Jstuelk