yxjF

yxjF
168

sporulation protein, 3-hydroxybutyrate dehydrogenase

Locus
BSU_38970
Molecular weight
27.46 kDa
Isoelectric point
7.89
Protein length
257 aaSequenceBlast
Gene length
774 bpSequenceBlast
Function
3-hydroxybutyrate utilization
Product
3-hydroxybutyrate dehydrogenase
Essential
no
Synonyms
yxjF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1028 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,000,539 → 4,001,312
The protein
Protein family
Short-chain dehydrogenases/reductases (SDR) family (according to UniProt)
Structure
2YZ7 (PDB) (from Alcaligenes Faecalis 42% identity)
P42317 (AlphaFold)
Paralogous protein(s)
YusZ
YxbG, (35%)
Expression and Regulation
Operons
Genes
hbuT-scoA-scoB-yxjF
Description
PubMed
Regulation
expressed early during sporulation in the mother cell (SigE) PubMed
Regulatory mechanism
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigE: sigma factor, PubMed, in sigE regulon
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hbuT→yxjF

2025-03-30 20:32:07

ghost

98

49dda0fadfba300315a7543307ab331d78e27e4b

9C2F2DF9D8BDF9896BE318E36ACFA3F55755DB86

Biological materials
Mutant
MGNA-B730 (yxjF::erm), available at the NBRP B. subtilis, Japan
GP1112 (spc), available in Stülke lab
BKE38970 (ΔyxjF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCAAACACCTTCCCAT,  downstream forward: _UP4_TGACATAAAAAACACACTTG
BKK38970 (ΔyxjF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCAAACACCTTCCCAT,  downstream forward: _UP4_TGACATAAAAAACACACTTG
References
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B7B1D28E50244704324920E5A6F110DA8D63F6C0

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Time of last update: 2025-04-09 01:30:21

Author of last update: Jstuelk