rpmI

rpmI
168

ribosomal protein bL35

Locus
BSU_28860
Molecular weight
7.42 kDa
Isoelectric point
12.52
Protein length
66 aaSequenceBlast
Gene length
201 bpSequenceBlast
Function
translation
Product
ribosomal protein L35 (bL35)
Essential
no
Synonyms
rpmI
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0291 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,952,615  2,952,815
The protein
Protein family
bacterial ribosomal protein bL35 family (single member, according to UniProt)
Structure
3J9W (PDB) (the ribosome) PubMed
P55874 (AlphaFold)
Additional information
the protein is significantly underrepresented in 45S assembly intermediates that accumulate upon depletion of RbgA PubMed
this ribosomal protein is lacking in some organisms with very small genomes PubMed
Expression and Regulation
Operons
Genes
infC-rpmI-rplT-ysdA
Description
PubMed
Regulation
Rel dependent downregulation (Class I) during stringent response PubMed
Regulatory mechanism
stringent response: negative regulation, in stringent response
RplT: termination, via binding to a RNA switch in the infC leader region causes transcription termination, in rplT regulon
Additional information
autorepression of infC-rpmI-rplT-ysdA expression upon binding of excess RplT to the untranslated region of the mRNA PubMed
expression of IF3 is uncoupled from that of L35 and L20 by RNase Y-dependent mRNA processing PubMed
the RNA switch RNA is degraded by RNase Y PubMed
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infC→ysdA

2025-03-17 20:13:15

Jstuelk

117

6ec080dce630885303f3f439404a1624accce660

33D5901765B267FCB4C316CA6BBC47536C5ACFC6

Biological materials
Mutant
BKE28860 (rpmI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGGATTTCCTCCTTATT,  downstream forward: _UP4_TAATCGGATAAGGAACAATT
BKK28860 (rpmI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGGATTTCCTCCTTATT,  downstream forward: _UP4_TAATCGGATAAGGAACAATT
References
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Time of last update: 2025-04-12 06:48:26

Author of last update: Jstuelk