epeE

epeE
168

radical SAM epimerase, modification of EpeX

Locus
BSU_40170
Molecular weight
37.06 kDa
Isoelectric point
5.86
Protein length
319 aaSequenceBlast
Gene length
960 bpSequenceBlast
Function
control of LiaR-LiaS activity
Product
radical SAM epimerase
Essential
no
Synonyms
yydG
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0535 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, AbrB regulon, Rok regulon

Gene
Coordinates
4,126,481  4,127,440
The protein
Catalyzed reaction/ biological activity
epimerization of Val-36 and Ile-44 of EpeX to the D-form PubMed
Protein family
radical SAM enzymes PubMed
Cofactors
two 4Fe-4S clusters PubMed
SAM PubMed
Structure
8AI1 (PDB) PubMed
Q45595 (AlphaFold)
Expression and Regulation
Operons
Genes
epeX-epeE-epeP-epeA-epeB
Description
PubMed
Regulation
repressed during logarithmic growth (AbrB) PubMed
Expression
expression is heterogeneous PubMed
Regulatory mechanism
AbrB: repression, PubMed, in abrB regulon
Rok: repression, PubMed, in rok regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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epeX→epeB

2025-04-03 14:38:08

Jstuelk

102

078981D5A4F256B0FA27157DFABCDDE534EC3306

BE1247045A413B93C3150C913C43B4E1B8098926

Biological materials
Mutant
MGNA-B810 (yydG::erm), available at the NBRP B. subtilis, Japan
BKE40170 (epeE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAAATATCCCCCAACAA,  downstream forward: _UP4_TATCCTTATATGGAAAAATA
BKK40170 (epeE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAAATATCCCCCAACAA,  downstream forward: _UP4_TATCCTTATATGGAAAAATA
References
Reviews
Gebhard S ABC transporters of antimicrobial peptides in Firmicutes bacteria - phylogeny, function and regulation. Molecular microbiology. 2012 Dec; 86(6):1295-317. doi:10.1111/mmi.12078. PMID:23106164
Original Publications
Kubiak X, Polsinelli I, Chavas LMG, Fyfe CD, Guillot A, Fradale L, Brewee C, Grimaldi S, Gerbaud G, Thureau A, Legrand P, Berteau O, Benjdia AStructural and mechanistic basis for RiPP epimerization by a radical SAM enzyme.Nature chemical biology. 2024 Mar; 20(3):382-391. PMID: 38158457
Mortier J, Van Riet S, Senovilla Herrero D, Vanoirbeek K, Aertsen AHigh-Throughput Time-Lapse Fluorescence Microscopy Screening for Heterogeneously Expressed Genes in Bacillus subtilis.Microbiology spectrum. 2022 Feb 23; 10(1):e0204521. PMID: 35171018
Popp PF, Friebel L, Benjdia A, Guillot A, Berteau O, Mascher TThe Epipeptide Biosynthesis Locus epeXEPAB Is Widely Distributed in Firmicutes and Triggers Intrinsic Cell Envelope Stress.Microbial physiology. 2021 Jun 11; :1-12. PMID: 34120110
Benjdia A, Guillot A, Ruffié P, Leprince J, Berteau O Post-translational modification of ribosomally synthesized peptides by a radical SAM epimerase in Bacillus subtilis. Nature chemistry. 2017 Jul; 9(7):698-707. doi:10.1038/nchem.2714. PMID:28644475
Salzberg LI, Powell L, Hokamp K, Botella E, Noone D, Devine KM The WalRK (YycFG) and σ(I) RsgI regulators cooperate to control CwlO and LytE expression in exponentially growing and stressed Bacillus subtilis cells. Molecular microbiology. 2013 Jan; 87(1):180-95. doi:10.1111/mmi.12092. PMID:23199363
Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. Molecular microbiology. 2011 Sep; 81(6):1459-73. doi:10.1111/j.1365-2958.2011.07777.x. PMID:21815947
Butcher BG, Lin YP, Helmann JD The yydFGHIJ operon of Bacillus subtilis encodes a peptide that induces the LiaRS two-component system. Journal of bacteriology. 2007 Dec; 189(23):8616-25. . PMID:17921301
Butcher BG, Lin YP, Helmann JD The yydFGHIJ operon of Bacillus subtilis encodes a peptide that induces the LiaRS two-component system. Journal of bacteriology. 2007 Dec; 189(23):8616-25. . PMID:17921301
Blencke HM, Homuth G, Ludwig H, Mäder U, Hecker M, Stülke J Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metabolic engineering. 2003 Apr; 5(2):133-49. . PMID:12850135

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Time of last update: 2025-04-04 03:03:05

Author of last update: Jstuelk