mrfB

mrfB
168

Mg2+-dependent 3'-5' DNA exonuclease, part of a novel nucleotide excision repair pathway (with MrfA)

Locus
BSU_22210
Molecular weight
47.83 kDa
Isoelectric point
7.16
Protein length
Gene length
Function
mitomycin C-specific DNA damage repair
Product
metal-dependent DNA exonuclease
Essential
no
Synonyms
mrfB, yprB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3359 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,333,324  2,334,565
Phenotypes of a mutant
sensitive to mitomycin C PubMed
The protein
Catalyzed reaction/ biological activity
repair of mitomycin C-induced DNA adducts PubMed
Mg2+ PubMed
Structure
Expression and Regulation
Operons
Description
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mrfAcotD

2025-01-15 22:19:23

Jstuelk

59

14a2bf6c0f2fb4d70fe21c4e69da61c2a7c1ca91

729017EE549CDEA9A9C262C8E1D54EEDD385E33D

Biological materials
Mutant
MGNA-A413 (yprB::erm), available at the NBRP B. subtilis, Japan
BKE22210 (mrfB::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_TAATGACATATCCGGCCTCC,  downstream forward: _UP4_TAAATATTCCCCGGGAAAGC
BKK22210 (mrfB::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_TAATGACATATCCGGCCTCC,  downstream forward: _UP4_TAAATATTCCCCGGGAAAGC
References
Reviews
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Research papers
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3F1D78CAAA4B5057DEBB24C4D382F96DA4AA4852

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Time of last update: 2025-01-17 03:37:49

Author of last update: Jstuelk