cheA

cheA
168

two-component sensor kinase, chemotactic signal modulator

Locus
BSU_16430
Molecular weight
74.59 kDa
Isoelectric point
4.45
Protein length
672 aaSequenceBlast
Gene length
2019 bpSequenceBlast
Function
chemotactic signal modulator
Product
two-component sensor kinase
Essential
no
Synonyms
cheA, cheN
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0643 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,712,815  1,714,833
Phenotypes of a mutant
not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed
defective in Swarming motility PubMed
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of CheY and CheB PubMed
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
Domains
HPt domain (aa 1-103) (according to UniProt)
P2/ CheY-binding domain
dimerization domain
Histidine kinase domain (aa 290-540) (according to UniProt)
CheW-like domain (aa 542-672) (according to UniProt)
Structure
6S1K (PDB) (from E. coli, 35% identity) PubMed
P29072 (AlphaFold)
Modification
autophosphorylation on a His residue
Effectors of protein activity
phosphorylation of CheA is stimulated by interaction with the complex of deaminated McpC-CheD PubMed
the kinase activity of CheA is activated by chemoreceptors bound to CheD PubMed
Localization
membrane (according to UniProt)
localizes to the cell poles PubMed
Expression and Regulation
Operons
Genes
ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliO-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
Description
PubMed
Regulation
see fla-che operon
Regulatory mechanism
Spo0A: repression, in spo0A regulon
CodY: repression, in codY regulon
DegU: repression, in degU regulon
SwrA: activation, in swrA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigD: sigma factor, PubMed, in sigD regulon
Open in new tab

ylxF→swrB

2025-04-04 21:21:07

Jstuelk

170

EECFE9022742ECC57D9BBA5CE7DB7A4D1BFD42A8

0535E3D13CBD000A94D62050515BF8D1D5E3F011

Genes
flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliO-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
Description
PubMed
Regulation
expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
Regulatory mechanism
Spo0A: repression, in spo0A regulon
SwrA: activation, in swrA regulon
CodY: repression, in codY regulon
DegU: repression, in degU regulon
SinR: transcription elongation, binding of the SinR-SlrR heteromer to sites within fliE and fliI results in inhibition of transcription elongation PubMed, in sinR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigD: sigma factor, PubMed, in sigD regulon
Open in new tab

flgB→swrB

2025-04-03 01:56:48

Jstuelk

173

024d59ae468aa14568753f57122b789c39ed6c06

4D920B925DA071AE7390E022B9AB625E40B7B2AC

Additional information
in minimal medium, CheA is present with 2,600 +/- 560 molecules per cell PubMed
Biological materials
Mutant
1A797 ( cheA::cat), PubMed, available at BGSC
DS6887 (marker-less in NCIB3610) PubMed
BKE16430 (cheA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATACTGATTCATATCCATTT,  downstream forward: _UP4_TAACCATTCGAGGAGGTGTC
BKK16430 (cheA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATACTGATTCATATCCATTT,  downstream forward: _UP4_TAACCATTCGAGGAGGTGTC
Labs working on this gene/protein
Daniel Kearns
References
Reviews
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Original Publications
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Time of last update: 2025-04-09 05:45:32

Author of last update: Jstuelk