iolG
168
inositol 2-dehydrogenase
Locus
BSU_39700
Molecular weight
38.20 kDa
Isoelectric point
4.86
Function
myo-inositol catabolism
Product
inositol 2-dehydrogenase
Essential
no
E.C.
1.1.1.18
Synonyms
iolG, idh, iol
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0673 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,075,785 4,076,819
The protein
Catalyzed reaction/ biological activity
myo-inositol + NAD+ --> H+ + NADH + scyllo-inosose (according to UniProt)
1D-chiro-inositol + NAD+ --> H+ + NADH + scyllo-inosine (according to UniProt)
Protein family
Gfo/Idh/MocA family (according to UniProt)
NAD+ PubMed
Structure
Paralogous protein(s)
Expression and Regulation
Operons
Biological materials
Mutant
MGNA-B698 (iolG::erm), available at the NBRP B. subtilis, Japan
Labs working on this gene/protein
Yasutaro Fujita, University of Fukuyama, Japan
[[Ken-ichi Yoshida]], Kobe University, Japan
References
Efficient pathway-driven scyllo-inositol production from myo-inositol using thermophilic cells and mesophilic inositol dehydrogenases: a novel strategy for pathway control.Applied and environmental microbiology. 2024 Jul 8; :e0028124. PMID: 38975762
. . PMID: 25019153
An improved Bacillus subtilis cell factory for producing scyllo-inositol, a promising therapeutic agent for Alzheimer's disease.
Microbial cell factories. 2013 Dec 11; 12:124. doi:10.1186/1475-2859-12-124. PMID:24325193
Converting NAD-specific inositol dehydrogenase to an efficient NADP-selective catalyst, with a surprising twist.
Biochemistry. 2013 Aug 27; 52(34):5876-83. doi:10.1021/bi400821s. PMID:23952058
Structural investigation of myo-inositol dehydrogenase from Bacillus subtilis: implications for catalytic mechanism and inositol dehydrogenase subfamily classification.
The Biochemical journal. 2010 Dec 01; 432(2):237-47. doi:10.1042/BJ20101079. PMID:20809899
myo-Inositol catabolism in Bacillus subtilis.
The Journal of biological chemistry. 2008 Apr 18; 283(16):10415-24. doi:10.1074/jbc.M708043200. PMID:18310071
Interaction of a repressor and its binding sites for regulation of the Bacillus subtilis iol divergon.
Journal of molecular biology. 1999 Jan 22; 285(3):917-29. . PMID:9887260
Organization and transcription of the myo-inositol operon, iol, of Bacillus subtilis.
Journal of bacteriology. 1997 Jul; 179(14):4591-8. . PMID:9226270
Catabolite repression of inositol dehydrogenase and gluconate kinase syntheses in Bacillus subtilis.
Biochimica et biophysica acta. 1984 Mar 22; 798(1):88-95. . PMID:6322857
Bacillus subtilis inositol dehydrogenase-encoding gene (idh): sequence and expression in Escherichia coli.
Gene. 1991 Dec 01; 108(1):121-5. . PMID:1761221
Purification and properties of Bacillus subtilis inositol dehydrogenase.
The Journal of biological chemistry. 1979 Aug 25; 254(16):7684-90. . PMID:112095
Purification and properties of Bacillus subtilis inositol dehydrogenase.
The Journal of biological chemistry. 1979 Aug 25; 254(16):7684-90. . PMID:112095
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Time of last update: 2025-01-21 13:27:19
Author of last update: Jstuelk