iolG

iolG
168

inositol 2-dehydrogenase

Locus
BSU_39700
Molecular weight
38.20 kDa
Isoelectric point
4.86
Protein length
Gene length
Function
myo-inositol catabolism
Product
inositol 2-dehydrogenase
Essential
no
E.C.
1.1.1.18
Synonyms
iolG, idh, iol

Genomic Context

List of homologs in different organisms, belongs to COG0673 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CcpA regulon, IolR regulon

Gene
Coordinates
4,075,785  4,076,819
The protein
Catalyzed reaction/ biological activity
myo-inositol + NAD+ --> H+ + NADH + scyllo-inosose (according to UniProt)
1D-chiro-inositol + NAD+ --> H+ + NADH + scyllo-inosine (according to UniProt)
Protein family
Gfo/Idh/MocA family (according to UniProt)
NAD+ PubMed
Structure
Paralogous protein(s)
Expression and Regulation
Operons
Description
Regulation
induced by inositol (IolR) PubMed
Regulatory mechanism
IolR: repression, PubMed, in iolR regulon
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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iolAiolJ

2025-01-11 21:13:47

ghost

110

4fd092965338ef1511600132442d2247a2693c98

C19E63046F0B412961D2626F72E0308D922D50B2

Biological materials
Mutant
MGNA-B698 (iolG::erm), available at the NBRP B. subtilis, Japan
BKE39700 (iolG::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTGACAGCCACTCCTTCT,  downstream forward: _UP4_AACTAAAAAACAGAGGAGTG
BKK39700 (iolG::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTGACAGCCACTCCTTCT,  downstream forward: _UP4_AACTAAAAAACAGAGGAGTG
Labs working on this gene/protein
Yasutaro Fujita, University of Fukuyama, Japan
[[Ken-ichi Yoshida]], Kobe University, Japan
References
Kurashiki R, Takahashi M, Okumura Y, Ono T, Endo H, Makino K, Fukui K, Yokoyama K, Ishikawa S, Yoshida K-i, Ohshiro T, Suzuki HEfficient pathway-driven scyllo-inositol production from myo-inositol using thermophilic cells and mesophilic inositol dehydrogenases: a novel strategy for pathway control.Applied and environmental microbiology. 2024 Jul 8; :e0028124. PMID: 38975762
. . PMID: 25019153
Tanaka K, Tajima S, Takenaka S, Yoshida K An improved Bacillus subtilis cell factory for producing scyllo-inositol, a promising therapeutic agent for Alzheimer's disease. Microbial cell factories. 2013 Dec 11; 12:124. doi:10.1186/1475-2859-12-124. PMID:24325193
Zheng H, Bertwistle D, Sanders DA, Palmer DR Converting NAD-specific inositol dehydrogenase to an efficient NADP-selective catalyst, with a surprising twist. Biochemistry. 2013 Aug 27; 52(34):5876-83. doi:10.1021/bi400821s. PMID:23952058
van Straaten KE, Zheng H, Palmer DR, Sanders DA Structural investigation of myo-inositol dehydrogenase from Bacillus subtilis: implications for catalytic mechanism and inositol dehydrogenase subfamily classification. The Biochemical journal. 2010 Dec 01; 432(2):237-47. doi:10.1042/BJ20101079. PMID:20809899
Yoshida K, Yamaguchi M, Morinaga T, Kinehara M, Ikeuchi M, Ashida H, Fujita Y myo-Inositol catabolism in Bacillus subtilis. The Journal of biological chemistry. 2008 Apr 18; 283(16):10415-24. doi:10.1074/jbc.M708043200. PMID:18310071
Yoshida KI, Shibayama T, Aoyama D, Fujita Y Interaction of a repressor and its binding sites for regulation of the Bacillus subtilis iol divergon. Journal of molecular biology. 1999 Jan 22; 285(3):917-29. . PMID:9887260
Yoshida KI, Aoyama D, Ishio I, Shibayama T, Fujita Y Organization and transcription of the myo-inositol operon, iol, of Bacillus subtilis. Journal of bacteriology. 1997 Jul; 179(14):4591-8. . PMID:9226270
Nihashi J, Fujita Y Catabolite repression of inositol dehydrogenase and gluconate kinase syntheses in Bacillus subtilis. Biochimica et biophysica acta. 1984 Mar 22; 798(1):88-95. . PMID:6322857
Fujita Y, Shindo K, Miwa Y, Yoshida K Bacillus subtilis inositol dehydrogenase-encoding gene (idh): sequence and expression in Escherichia coli. Gene. 1991 Dec 01; 108(1):121-5. . PMID:1761221
Ramaley R, Fujita Y, Freese E Purification and properties of Bacillus subtilis inositol dehydrogenase. The Journal of biological chemistry. 1979 Aug 25; 254(16):7684-90. . PMID:112095
Ramaley R, Fujita Y, Freese E Purification and properties of Bacillus subtilis inositol dehydrogenase. The Journal of biological chemistry. 1979 Aug 25; 254(16):7684-90. . PMID:112095

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Time of last update: 2025-01-21 13:27:19

Author of last update: Jstuelk