Difference between revisions of "PGP574"
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| − | + | Vector for the expression of proteins in ''E. coli'', the [[plasmids|plasmid]] allows to fuse a Strep-tag to the C-terminus of the expressed protein | |
| − | + | host: ''E. coli'' BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | |
| − | |||
| − | host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | ||
constructed in the [[Stülke]] lab | constructed in the [[Stülke]] lab | ||
| − | in E. coli: ampicillin resistance | + | in ''E. coli'': ampicillin resistance |
based on pET3C | based on pET3C | ||
| − | similar plasmid: [[pGP172]] | + | similar [[plasmid]]: [[pGP172]] |
| + | |||
| + | * sequencing primer: | ||
| + | **'''T7:''' 5’-TAATACGACTCACTATAGGG -3’ | ||
| + | ** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’ | ||
| − | + | <pubmed> 17074746 </pubmed> | |
| + | == Return to [[Plasmids]] == | ||
Latest revision as of 14:04, 3 June 2013
Vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the C-terminus of the expressed protein
host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Stülke lab
in E. coli: ampicillin resistance
based on pET3C
- sequencing primer:
- T7: 5’-TAATACGACTCACTATAGGG -3’
- NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
Oliver Schilling, Christina Herzberg, Tina Hertrich, Hanna Vörsmann, Dirk Jessen, Sebastian Hübner, Fritz Titgemeyer, Jörg Stülke
Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples.
Nucleic Acids Res: 2006, 34(21);6102-15
[PubMed:17074746]
[WorldCat.org]
[DOI]
(I p)
