yacO

yacO
168

putative 23S rRNA 2'-O-ribose G2251 methyltransferase

Locus
BSU_00960
Molecular weight
27.36 kDa
Isoelectric point
8.63
Protein length
249 aaSequenceBlast
Gene length
750 bpSequenceBlast
Function
rRNA modification
Product
putative 23S rRNA methyltransferase
Essential
no
E.C.
2.1.1.-
Synonyms
yacO, rlmB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0566 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
115,269  116,018
The protein
Protein family
Methyltransferase superfamily (according to UniProt)
class IV-like SAM-binding methyltransferase superfamily (with CspR and RlmP, according to UniProt)
Cofactors
SAM (according to UniProt)
Structure
1GZ0 (PDB) (from E. coli, 44% identity) PubMed
Q06753 (AlphaFold)
Paralogous protein(s)
RlmP
Localization
cytoplasm (according to UniProt)
Expression and Regulation
Operons
Genes
gltX-cysE-cysS-mrnC-yacO-rae1
Description
PubMed
Regulation
expression transiently increases in the forespore PubMed
the T-box RNA is degraded by RNase Y PubMed
Regulatory mechanism
T-box: RNA switch, overlaps a transcription terminator upstream of cysE, in T-box
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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gltX→rae1

2025-04-04 01:30:11

Jstuelk

96

9ab774439b3983729fe5811aeaf44f4bc16c609f

BCC6FA512C81F405B92E34AE44A52F949D245F3D

Biological materials
Mutant
MGNA-B884 (yacO::erm), available at the NBRP B. subtilis, Japan
BKE00960 (yacO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTGCATTTTTCCCTATGA,  downstream forward: _UP4_AACCCTGTGGGAGAATAAAG
BKK00960 (yacO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTGCATTTTTCCCTATGA,  downstream forward: _UP4_AACCCTGTGGGAGAATAAAG
References
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Time of last update: 2025-04-08 14:07:02

Author of last update: Jstuelk