mleA

mleA
168

malic enzyme

Locus
BSU_23550
Molecular weight
45.73 kDa
Isoelectric point
4.89
Protein length
439 aaSequenceBlast
Gene length
1320 bpSequenceBlast
Function
malate utilization
Product
NAD+-dependent malate dehydrogenase
Essential
no
E.C.
1.1.1.38
Synonyms
mleA, yqkJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0281 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,451,463  2,452,782
The protein
Catalyzed reaction/ biological activity
(S)-malate + NAD+ --> CO2 + NADH + pyruvate (according to UniProt)
H+ + oxaloacetate --> CO2 + pyruvate (according to UniProt)
Protein family
malic enzymes family (according to UniProt)
Domains
ACT domain (aa 9-84) (according to UniProt)
Structure
2A9F (PDB) (from Streptococcus pneumoniae, 42% identity from aa 90 - 435)
P54572 (AlphaFold)
Paralogous protein(s)
YtsJ, MalS
Expression and Regulation
Operons
Genes
mleN-mleA
Description
PubMed
Regulation
''ansA'': expressed in the presence of asparagine (AnsR) PubMed
Regulatory mechanism
AnsR: repression, PubMed, in ansR regulon
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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mleN→mleA

2025-04-09 16:45:59

ghost

74

2adb475a1d359d8484e1d13c3462a134569ab20a

12E51CE78001847EEC34BF1A5209896CF1E02F0D

Genes
ansA-ansB-mleN-mleA
Description
PubMed
Regulation
AnsA: expressed in the presence of asparagine (AnsR) PubMed
Expression
expressed in the presence of asparagine (AnsR) PubMed
expression is reduced in motile cells as compared to non-motile cells PubMed
Regulatory mechanism
AnsR: transcription repression, PubMed, in ansR regulon
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ansA→mleA

2025-04-04 08:53:37

Jstuelk

102

6E6CC6ADE2F20EBE93C9F1ED4C2EF4E87AF126B1

D7F0E184034763E326DE2841E35068D2FD73DD5D

Biological materials
Mutant
MGNA-C412 (yqkJ::erm), available at the NBRP B. subtilis, Japan
GP1136 (cat) available in Jörg Stülke's lab
BKE23550 (mleA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TATCATTCAGTGATCCTCCC,  downstream forward: _UP4_TAAATGTAGCTAAACAGCAC
BKK23550 (mleA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TATCATTCAGTGATCCTCCC,  downstream forward: _UP4_TAAATGTAGCTAAACAGCAC
References
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160EBB7885D7C7FD30A6E35605A862C156E2DA80

Page visits: 4808

Time of last update: 2025-04-21 04:53:24

Author of last update: Jstuelk