Difference between revisions of "PETM-11"
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* vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease | * vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease | ||
| − | |||
* host: ''E. coli'' BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | * host: ''E. coli'' BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | ||
| − | |||
* in ''E. coli'': ampicillin resistance | * in ''E. coli'': ampicillin resistance | ||
| − | + | *constructed by EMBL | |
| − | * | ||
*sequencing primer: | *sequencing primer: | ||
| − | *'''CZ103''' | + | *'''CZ103''': 5’-GAGGATCGAGATCTCGATC-3‘ |
*'''CZ104''': 5’-CTAGTTATTGCTCAGCGGTG-3‘ | *'''CZ104''': 5’-CTAGTTATTGCTCAGCGGTG-3‘ | ||
| + | <pubmed> 16351710 </pubmed> | ||
| − | |||
== Return to [[Plasmids]] == | == Return to [[Plasmids]] == | ||
Latest revision as of 10:00, 19 November 2015
- vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease
- host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
- in E. coli: ampicillin resistance
- constructed by EMBL
- sequencing primer:
- CZ103: 5’-GAGGATCGAGATCTCGATC-3‘
- CZ104: 5’-CTAGTTATTGCTCAGCGGTG-3‘
