Rnz

• Description: RNase Z
  
  

Gene name	rnz
Synonyms	yqjK
Essential	yes PubMed
Product	endoribonuclease Z
Function	processing of CCA-less tRNA precursors
Gene expression levels in SubtiExpress: rnz
MW, pI	33 kDa, 5.805
Gene length, protein length	921 bp, 307 aa
Immediate neighbours	rpmGC, zwf
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

Rnases, translation, essential genes

The gene

Basic information

• Locus tag: BSU23840

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU23840

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs (according to Swiss-Prot)
  • 3' end maturation of tmRNA PubMed

• Protein family: RNase Z family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU23840

• Structure: 2FK6 (in complex with tRNA-Thr) PubMed, 1Y44

• UniProt: P54548

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rnz (according to DBTBS)

• Expression browser: rnz PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 55 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 278 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 379 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 311 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Ciaran Condon, IBPC, Paris, France Homepage

References

Reviews

Murray P Deutscher
How bacterial cells keep ribonucleases under control.
FEMS Microbiol Rev: 2015, 39(3);350-61
[PubMed:25878039] [WorldCat.org] [DOI] (I p)

Roland K Hartmann, Markus Gössringer, Bettina Späth, Susan Fischer, Anita Marchfelder
The making of tRNAs and more - RNase P and tRNase Z.
Prog Mol Biol Transl Sci: 2009, 85;319-68
[PubMed:19215776] [WorldCat.org] [DOI] (P p)

B Späth, G Canino, A Marchfelder
tRNase Z: the end is not in sight.
Cell Mol Life Sci: 2007, 64(18);2404-12
[PubMed:17599240] [WorldCat.org] [DOI] (P p)