Difference between revisions of "PGP1370"
(Created page with "'''Application:''' The vector was constructed in the Stülke lab and it is suitable for fusion of C-terminal 3xFLAG-tag to the protein of interest in ''B. subtilis''. The pla...") |
|||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
'''Application:''' | '''Application:''' | ||
| − | The vector was constructed in | + | The vector was constructed in [[Jörg Stülke]]'s lab and it is suitable for fusion of C-terminal 3xFLAG-tag to the protein of interest in ''B. subtilis''. The plasmid confers resistance to ampicillin and erythromycin in ''E. coli'' and ''B. subtilis'', respectively. [[pGP1370]] is based on the vector [[pBQ200]]. A Shine-Dalgarno sequence has to be fused to the open reading frame during PCR. |
Sequencing primers: | Sequencing primers: | ||
| Line 8: | Line 8: | ||
*'''FX125 (rev; primes -85bp of MCS):''' 5‘-GGCTCGTATGTTGTGTGG-3‘ | *'''FX125 (rev; primes -85bp of MCS):''' 5‘-GGCTCGTATGTTGTGTGG-3‘ | ||
*'''JN54 (fwd; primes +63bp of MCS):''' 5‘-GTGAAAAATGAGCCGAAAGCAG-3‘ | *'''JN54 (fwd; primes +63bp of MCS):''' 5‘-GTGAAAAATGAGCCGAAAGCAG-3‘ | ||
| + | |||
| + | '''The reference''' | ||
| + | <pubmed>21803996</pubmed> | ||
| + | == Return to [[Plasmids]] == | ||
Latest revision as of 14:13, 24 November 2014
Application:
The vector was constructed in Jörg Stülke's lab and it is suitable for fusion of C-terminal 3xFLAG-tag to the protein of interest in B. subtilis. The plasmid confers resistance to ampicillin and erythromycin in E. coli and B. subtilis, respectively. pGP1370 is based on the vector pBQ200. A Shine-Dalgarno sequence has to be fused to the open reading frame during PCR.
Sequencing primers:
- M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
- M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘
- FX125 (rev; primes -85bp of MCS): 5‘-GGCTCGTATGTTGTGTGG-3‘
- JN54 (fwd; primes +63bp of MCS): 5‘-GTGAAAAATGAGCCGAAAGCAG-3‘
The reference
Martin Lehnik-Habrink, Joseph Newman, Fabian M Rothe, Alexandra S Solovyova, Cecilia Rodrigues, Christina Herzberg, Fabian M Commichau, Richard J Lewis, Jörg Stülke
RNase Y in Bacillus subtilis: a Natively disordered protein that is the functional equivalent of RNase E from Escherichia coli.
J Bacteriol: 2011, 193(19);5431-41
[PubMed:21803996]
[WorldCat.org]
[DOI]
(I p)
