rph

rph
168

RNase PH, 3-5 exoribonuclease

Locus
BSU_28370
Molecular weight
26.53 kDa
Isoelectric point
4.89
Protein length
Gene length
Function
3-5 exoribonuclease
Product
RNase PH (EC 2.7.7.56)
Essential
no
E.C.
2.7.7.56
Synonyms
rph

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0689 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,901,154  2,901,891
The protein
Catalyzed reaction/ biological activity
major player in the exonucleolytic maturation of CCA-containing tRNA precursors PubMed
responsible for endonucleolytic cleavage of scRNA (''scr'') PubMed
phosphate + tRNA(n+1) --> ribonucleoside 5'-diphosphate + tRNA(n) (according to UniProt)
Protein family
RNase PH family (single member, according to UniProt)
Structure
Expression and Regulation
Operons
Description
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rphysnB

2025-02-19 23:05:00

Jstuelk

59

0f9e962c8f46d37722865610c6c0a69f06780cd3

B6C3C0619B9997D38F45FCD64F603B8C04463A79

Biological materials
Mutant
BKE28370 (rph::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATGTTTTACCTCCGATTGT,  downstream forward: _UP4_TAAAGACAGAAAGGCTTGAT
BKK28370 (rph::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATGTTTTACCTCCGATTGT,  downstream forward: _UP4_TAAAGACAGAAAGGCTTGAT
Labs working on this gene/protein
David Bechhofer, Mount Sinai School, New York, USA Homepage
Ciaran Condon, IBPC, Paris, France Homepage
References
Reviews
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Original Publications
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A18474CD50C2A1A83CF04C74098659BF1649B4DC

Page visits: 3075

Time of last update: 2025-02-22 04:44:58

Author of last update: Jstuelk