Difference between revisions of "SepF"
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| − | * '''Description:''' part of the divisome, recruits [[FtsZ]] to the membrane <br/><br/> | + | * '''Description:''' part of the [[divisome]], recruits [[FtsZ]] to the membrane <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15390 sepF] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15390 sepF] | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=SepF SepF] |
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.863 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.863 | ||
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* less efficient cell division results in longer cells. Electron microscopy reveals strongly distorted division septa. | * less efficient cell division results in longer cells. Electron microscopy reveals strongly distorted division septa. | ||
* the ''[[sepF]]'' mutation in combination with a constitutively active form of [[WalR]] ([[WalR]]-R204C) results in the formation of cell wall-less L-forms {{PubMed|22122227}} | * the ''[[sepF]]'' mutation in combination with a constitutively active form of [[WalR]] ([[WalR]]-R204C) results in the formation of cell wall-less L-forms {{PubMed|22122227}} | ||
| − | * the ''sepF'' mutation is synthetically lethal in combination with an ''[[ezrA]]'' mutation or an ''[[ftsA]]'' mutation | + | * the ''sepF'' mutation is synthetically lethal in combination with an ''[[ezrA]]'' mutation or an ''[[ftsA]]'' mutation {{PubMed|24218584}} |
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15390&redirect=T BSU15390] | ||
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ylmDEFGH.html] | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ylmDEFGH.html] | ||
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* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
** SepF assembles into very large (∼50 nm diameter) rings. These rings are able to bundle [[FtsZ]] protofilaments into strikingly long and regular tubular structures reminiscent of eukaryotic microtubules {{PubMed|21224850}} | ** SepF assembles into very large (∼50 nm diameter) rings. These rings are able to bundle [[FtsZ]] protofilaments into strikingly long and regular tubular structures reminiscent of eukaryotic microtubules {{PubMed|21224850}} | ||
| + | ** SepF anchors [[FtsZ]] bundles to the membrane {{PubMed|24218584}} | ||
* '''Protein family:''' sepF family (according to Swiss-Prot) | * '''Protein family:''' sepF family (according to Swiss-Prot) | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
** N-terminal amphipatic helix for membrane binding {{PubMed|24218584}} | ** N-terminal amphipatic helix for membrane binding {{PubMed|24218584}} | ||
| − | ** C-terminal [[FtsZ]]-binding domain {{PubMed|24218584}} | + | ** C-terminal globular [[FtsZ]]-binding domain {{PubMed|24218584}} |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| + | ** forms filaments that are made up of dimers {{PubMed|24218584}} | ||
** [[FtsZ]] (extreme C terminus of [[FtsZ]])-[[SepF]] {{PubMed|24218584,22912848,16420366}} | ** [[FtsZ]] (extreme C terminus of [[FtsZ]])-[[SepF]] {{PubMed|24218584,22912848,16420366}} | ||
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15390&redirect=T BSU15390] | ||
* '''Structure:''' | * '''Structure:''' | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sepF_1610865_1611314_1 sepF] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sepF_1610865_1611314_1 sepF] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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* '''Additional information:''' | * '''Additional information:''' | ||
| + | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 437 {{PubMed|24696501}} | ||
| + | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 837 {{PubMed|24696501}} | ||
=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' | + | * '''Mutant:''' |
| + | ** YK204 (''[[sepF]]''::''spc''), available in the labs of ''Leendert Hamoen'' and ''Jörg Stülke'' | ||
* '''Expression vector:''' | * '''Expression vector:''' | ||
Latest revision as of 12:43, 22 May 2014
| Gene name | sepF |
| Synonyms | ylmF |
| Essential | no |
| Product | FtsZ-interacting protein |
| Function | recruitment of FtsZ |
| Gene expression levels in SubtiExpress: sepF | |
| Interactions involving this protein in SubtInteract: SepF | |
| MW, pI | 17 kDa, 4.863 |
| Gene length, protein length | 447 bp, 149 aa |
| Immediate neighbours | ylmE, ylmG |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell division, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU15390
Phenotypes of a mutant
- perturbation of the formation of properly formed division septa
- less efficient cell division results in longer cells. Electron microscopy reveals strongly distorted division septa.
- the sepF mutation in combination with a constitutively active form of WalR (WalR-R204C) results in the formation of cell wall-less L-forms PubMed
- the sepF mutation is synthetically lethal in combination with an ezrA mutation or an ftsA mutation PubMed
Database entries
- BsubCyc: BSU15390
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: sepF family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- BsubCyc: BSU15390
- UniProt: O31728
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- YK204 (sepF::spc), available in the labs of Leendert Hamoen and Jörg Stülke
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Leendert Hamoen, CBCB, Newcastle University, UK
Shu Ishikawa, Nara Institute of Science and Technology, Nara, Japan
Your additional remarks
SepF mutation is synthetic lethal in combination with an ezrA mutation or an ftsA mutation.
References
Reviews
Original Publications
