bacD

bacD
168

alanine-anticapsin ligase

Locus
BSU_37710
Molecular weight
52.10 kDa
Isoelectric point
4.68
Protein length
Gene length
Function
biosynthesis of the antibiotic bacilysin
Product
alanine-anticapsin ligase
Essential
no
E.C.
6.3.2.28
Synonyms
bacD, ywfE, ipa-83d

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0439 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,870,668  3,872,086
Phenotypes of a mutant
a bacD point mutation has been isolated upon selction at low pressure PubMed
The protein
Catalyzed reaction/ biological activity
ligation of anticapsin to L-Ala to form bacilysin, ultimate step in bacilysin biosynthesis PubMed
ATP + L-alanine + L-anticapsin --> ADP + bacilysin + H+ + phosphate (according to UniProt)
ATP-grasp doamin (aa 142-355) (according to UniProt)
Structure
Expression and Regulation
Operons
Description
Regulation
heterogeneous expression, percentage of expressing cells increases during colonization of plant roots PubMed
Regulatory mechanism
CodY: repression, PubMed, in codY regulon
AbrB: repression, PubMed, in abrB regulon
ScoC: repression, PubMed, in scoC regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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bacAbacF

2025-02-25 11:07:10

Jstuelk

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7D644717829C1A41F65E52A4312081846FB55951

33F4BF1A14F6AC22A6ACC7091140A7F012E3837C

Biological materials
Mutant
MGNA-B241 (ywfE::erm), available at the NBRP B. subtilis, Japan
BKE37710 (bacD::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CTCCATATGTAAAACACTCC,  downstream forward: _UP4_ACGGCAAAGTATGTGCTGCC
BKK37710 (bacD::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CTCCATATGTAAAACACTCC,  downstream forward: _UP4_ACGGCAAAGTATGTGCTGCC
References
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Page visits: 4748

Time of last update: 2025-02-28 06:07:35

Author of last update: Melvin.boenninger