Difference between revisions of "PAC6"

Latest revision as of 13:53, 3 June 2013

pAC6: click to enlarge

vector for the construction of transcriptional lacZ fusions that can be integrated at the amyE site

does not replicate in B. subtilis, integrates into the amyE site

constructed in the Rapoport lab

in E. coli: ampicillin resistance

in B. subtilis: chloramphenicol resistance

similar vectors: pAC5, pAC7

sequencing primer:

@@ Line 1: / Line 1: @@
-pAC6
+[[File:PAC6.jpeg|250px|thumb|right|'''pAC6: click to enlarge''']]
 vector for the construction of transcriptional ''lacZ'' fusions that can be integrated at the ''amyE'' site
@@ Line 7: / Line 7: @@
 constructed in the Rapoport lab
-in E. coli: ampicillin resistance
+in ''E. coli'': ampicillin resistance
-in B. subtilis: chloramphenocol resistance
+in ''B. subtilis'': chloramphenicol resistance
 similar vectors: [[pAC5]], [[pAC7]]
-Martin-Verstraete I, Débarbouillé M, Klier A, Rapoport G: (1992) Mutagenesis of the Bacillus subtilis ‘-12, -24’ promoter of the levanase operon and evidence for the existence of an upstream activating sequence. J Mol Biol , 226:85-99. [http://www.ncbi.nlm.nih.gov/sites/entrez/1619665  PubMed]
+sequencing primer:
+*'''PAC5R:''' 5’-CTGCAAGCGATAAGTTGG-3’
+*'''PAC5F:''' 5’-GCGTAGCGAAAAATCCTTTTC-3‘
+*'''Catcheckrev:''' 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’
+<pubmed> 11902727 </pubmed>
+==  Return to [[Plasmids]] ==