Difference between revisions of "PGP570"
(New page: vector for constructed in the --- lab in E. coli: ampicillin resistance in B. subtilis: ---- resistance based on --- reference. PubMed) |
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| − | + | Vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a His(6) tag to the N-terminus of the expressed protein, after purification the tag can be cleaved off by thrombin | |
| − | constructed in the | + | |
| − | in E. coli: ampicillin resistance | + | the [[plasmid]] carries the ''lacI'' gene, regulation is possible in any ''E. coli'' strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture |
| − | + | ||
| − | + | constructed in the [[Stülke]] lab | |
| − | + | ||
| + | based on [[pWH844]] | ||
| + | |||
| + | in ''E. coli'': ampicillin resistance | ||
| + | |||
| + | similar [[list of plasmids|plasmid]]: [[pWH844]] | ||
| + | |||
| + | unpublished | ||
| + | == Return to [[Plasmids]] == | ||
Latest revision as of 14:03, 3 June 2013
Vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein, after purification the tag can be cleaved off by thrombin
the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Stülke lab
based on pWH844
in E. coli: ampicillin resistance
unpublished
