splB
168
spore photoproduct lyase, radical SAM enzyme
Locus
BSU_13930
Molecular weight
39.79 kDa
Isoelectric point
8.52
Function
protection of spore DNA against photodamage
Product
spore photoproduct lyase
Essential
no
Synonyms
splB, spl
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1533 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,461,770 → 1,462,798
Phenotypes of a mutant
sensitive to blue light-induced DNA damage PubMed
The protein
Catalyzed reaction/ biological activity
repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase
(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine in DNA --> thymidine dimer in DNA (according to UniProt)
Protein family
Radical SAM superfamily (according to UniProt)
Fe-S cluster PubMed
uses the [4Fe-4S] cluster to reduce SAM
Structure
4FHC (PDB) (SplB from Geobacillus thermodenitrificans, 72% identity, 90% similarity) PubMed
Expression and Regulation
Operons
Description
Regulation
Regulatory mechanism
SplA: negative autoregulation, in splA regulon
Sigma factors
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Biological materials
Mutant
References
Reviews
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Original Publications
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Structure of the spore photoproduct lesion in DNA
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Time of last update: 2025-02-25 19:28:32
Author of last update: Jstuelk