splB

splB
168

spore photoproduct lyase, radical SAM enzyme

Locus
BSU_13930
Molecular weight
39.79 kDa
Isoelectric point
8.52
Protein length
Gene length
Function
protection of spore DNA against photodamage
Product
spore photoproduct lyase
Essential
no
Synonyms
splB, spl

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1533 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,461,770 → 1,462,798
Phenotypes of a mutant
sensitive to blue light-induced DNA damage PubMed
The protein
Catalyzed reaction/ biological activity
repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase
(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine in DNA --> thymidine dimer in DNA (according to UniProt)
Protein family
Radical SAM superfamily (according to UniProt)
Fe-S cluster PubMed
uses the [4Fe-4S] cluster to reduce SAM
Structure
4FHC (PDB) (SplB from Geobacillus thermodenitrificans, 72% identity, 90% similarity) PubMed
Expression and Regulation
Operons
Genes
Description
Regulation
expressed during sporulation in the forespore (SigG) PubMed
Regulatory mechanism
SplA: negative autoregulation, in splA regulon
Sigma factors
SigG: sigma factor, PubMed, in sigG regulon
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splAsplB

2025-02-23 11:31:21

ghost

87

05c0d6ddc5311aaa4612ef8b425cad6eec3b8cd4

ADCA4EE37BF8E4BFD653F8A03FBE5F3F554C5DF8

Biological materials
Mutant
BP130 (ΔsplB::spc), available in Fabian Commichau's and Jörg Stülke's labs PubMed
BKE13930 (ΔsplB::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATGATGCCACATCCTTTTC,  downstream forward: _UP4_TTCACTTAAACGGGCTGTTG
BKK13930 (ΔsplB::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATGATGCCACATCCTTTTC,  downstream forward: _UP4_TTCACTTAAACGGGCTGTTG
References
Reviews
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Original Publications
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Structure of the spore photoproduct lesion in DNA
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B873AE29ACAA0714D5F2E8648449B3280353C8AA

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Time of last update: 2025-02-25 19:28:32

Author of last update: Jstuelk