rpoY

rpoY
168

epsilon subunit of RNA polymerase

Locus
BSU_14540
Molecular weight
8.12 kDa
Isoelectric point
4.61
Protein length
69 aaSequenceBlast
Gene length
210 bpSequenceBlast
Function
control of RNA polymerase activity
Product
epsilon subunit of RNA polymerase
Essential
no
Synonyms
rpoY, ykzG
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5503 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,524,791  1,525,000
The protein
Protein family
UPF0356 family (single member, according to UniProt)
Structure
4NJC (PDB) PubMed
O31718 (AlphaFold)
Localization
colocalizes with the nucleoid PubMed
Expression and Regulation
Operons
Genes
rpoY-rnjA
Description
PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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rpoY→rnjA

2025-03-25 09:59:52

Bzhu

149

06939a764aef4750677f67254bb2b47e1f243fba

AED6E5D8624540AD5B702F0D49459F8B90D42090

Biological materials
Mutant
BKE14540 (rpoY::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCAAATCTCTCCTTAA,  downstream forward: _UP4_AAAGTATTGGAGTTATGAGC
BKK14540 (rpoY::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCAAATCTCTCCTTAA,  downstream forward: _UP4_AAAGTATTGGAGTTATGAGC
Expression vectors
pGP2824: expression in B. subtilis, with C-terminal Strep-tag, in pGP382, available in Jörg Stülke's lab
LacZ fusion
pGP420 (in pAC7), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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5F41273FB867C688011738CD45AA6FF5D3987EFE

Page visits: 3344

Time of last update: 2025-04-09 18:04:36

Author of last update: Jstuelk