ccpN

ccpN
168

transcriptional repressor (CcpN family) of gluconeogenetic genes and of sr1, repression in the presence of glucose, involved in the control of cell elongation

Locus
BSU_25250
Molecular weight
23.72 kDa
Isoelectric point
7.22
Protein length
212 aaSequenceBlast
Gene length
639 bpSequenceBlast
Function
repressor of genes involved in gluconeogenesis (gapB, pckA) and of sr1
Product
transcriptional regulator (CcpN family)
Essential
no
Synonyms
ccpN, yqzB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2905 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,604,959  2,605,597
Phenotypes of a mutant
Impaired growth on glucose due to re-routing of carbon from glycolysis to the pentose phosphate pathway PubMed
The protein
Catalyzed reaction/ biological activity
transcription repression of the gapB, pckA, and sr1 genes in the presence of glucose PubMed
Protein family
CcpN family
Domains
HTH deoR-type domain (aa 6-70) (according to UniProt)
2 CBS domains (aa 83-139, aa 148-211) (according to UniProt)
Structure
3FV6 (PDB) (the CBS domains, aa 63 ... 212)
O34994 (AlphaFold)
Effectors of protein activity
YqfL (negative effector of CcpN activity) PubMed
ATP enhances CcpN-dependent repression, ADP counteracts the repressing activity of CcpN PubMed
Expression and Regulation
Operons
Genes
ccpN-yqfL
Description
PubMed
Regulation
constitutive PubMed
Additional information
the intracellular concentration of CcpN is about 4 myM (according to PubMed).
Open in new tab

ccpN→yqfL

2025-04-08 16:20:44

ghost

99

3fe4ca7e20abf4f06c8caf7d6e2bf664f557186b

0E37F61E4E6EAE016A7E0E8F6D82E3B6339F8D91

Biological materials
Mutant
GP3407 (ΔccpN::kan) , available in Jörg Stülke's lab
GP1678 (Δ(ccpN-yqfL::aphA3 trpC2), available in Jörg Stülke's lab
MGNA-C493 (yqzB::erm), available at the NBRP B. subtilis, Japan
DB104 ccpN::cat, available in Sabine Brantl's lab
GP1128 ccpN::cat, available in Jörg Stülke's lab
BKE25250 (ccpN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCACCTTTTCACTTCATA,  downstream forward: _UP4_TAAGATTGCAAACTAACGGG
BKK25250 (ccpN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCACCTTTTCACTTCATA,  downstream forward: _UP4_TAAGATTGCAAACTAACGGG
Expression vectors
pGP2923 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
Antibody
available in Sabine Brantl's lab
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage
Uwe Sauer, ETH Zrich, Switzerland homepage
References
Reviews
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Original Publications
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2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636

Page visits: 6936

Time of last update: 2025-04-22 07:30:28

Author of last update: Jstuelk