spoIIE

spoIIE
168

protein serine phosphatase, septum-associated PP2C, dephosphorylation of SpoIIAA, required for the localization of FtsA-FtsZ on the mother cell side of the invaginating sporulation septa

Locus
BSU_00640
Molecular weight
91.78 kDa
Isoelectric point
6.14
Protein length
827 aaSequenceBlast
Gene length
2484 bpSequenceBlast
Function
control of SigF activity, required for normal formation of the asymmetric septum
Product
protein serine phosphatase, septum-associated PP2C
Essential
no
E.C.
3.1.3.16
Synonyms
spoIIE, spoIIH, spoIIK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5817 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
70,538  73,021
Phenotypes of a mutant
delay of the formation of polar FtsZ rings during sporulation PubMed
The protein
Catalyzed reaction/ biological activity
dephosphorylation of SpoIIAA (according to UniProt)
stabilization of ftsZ oligomers PubMed
required for the localization of FtsA-FtsZ on the mother cell side of the invaginating sporulation septa PubMed
Protein family
PP2C phosphatase
Domains
N-terminal trans-membrane domain PubMed
central oligomerization and FtsZ interaction domain PubMed
C-terminal PP2C phosphatase domain PubMed
PPM-type phosphatase domain (aa 594-804) (according to UniProt)
Cofactors
Mn 2+ PubMed
Structure
5UCG (PDB) PubMed
3T91 (PDB) (phosphatase domain) PubMed
P37475 (AlphaFold)
Effectors of protein activity
degraded by FtsH, oligomerization and polar localization protect SpoIIE from degradation PubMed
Localization
forespore face of the polar septum PubMed
cell membrane PubMed
at the side of asymmetric septum formation PubMed
localizes to the polar cell division sites where it causes FtsZ to relocate from mid-cell to form polar Z-rings
forespore  PubMed
localizes to the cell poles (via DivIVA) PubMed
proper localization in the engulfing membrane of the foreshore and stability depend on SpoIIQ PubMed
Expression and Regulation
Operons
Genes
spoIIE
Description
(DBTBS) null
Regulation
expressed under conditions that trigger sporulation (Spo0A) PubMed
Regulatory mechanism
Spo0A: activation, PubMed, in spo0A regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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spoIIE

2025-04-04 02:43:59

ghost

87

83eff014f20e329e8ab20d546c1d1fed8b41ed3a

2F87975B32F8872567520DAC684A083BD5AF3143

Genes
spoIIE-yabS-yabT-tilS-hprT-ftsH
Description
PubMed
Regulation
expressed early during sporulation in the forespore (SigF) PubMed
Sigma factors
SigF: sigma factor, PubMed, in sigF regulon
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spoIIE->ftsH

2025-04-03 08:25:04

Jstuelk

122

7069328a463a18ae1e3c27f03ddf76f79efe7c06

8FBB4B976CF46DAD9437300BBE1B22B618D33ED0

Biological materials
Mutant
MGNA-A083 (spoIIE::erm), available at the NBRP B. subtilis, Japan
BKE00640 (spoIIE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCTCTCATCTCCCACC,  downstream forward: _UP4_TAACGCTTCCGTATAAATCA
BKK00640 (spoIIE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCTCTCATCTCCCACC,  downstream forward: _UP4_TAACGCTTCCGTATAAATCA
Labs working on this gene/protein
Imrich Barak, Slovak Academy of Science, Bratislava, Slovakia homepage
References
Reviews
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Original Publications
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7C8DFE00A2B2B30CC8BEB35055D92CC2E4128F3A

Page visits: 9133

Time of last update: 2025-04-06 13:36:18

Author of last update: Jstuelk