guaB

guaB
168

IMP dehydrogenase

Locus
BSU_00090
Molecular weight
52.82 kDa
Isoelectric point
6.17
Protein length
488 aaSequenceBlast
Gene length
1467 bpSequenceBlast
Function
biosynthesis of GMP
Product
IMP dehydrogenase
Essential
no
E.C.
1.1.1.205
Synonyms
guaB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0517 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
15,915 17,381
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth PubMed
essential PubMed, non-essential according to PubMed
reduced expression of guaA suppresses the requirement of a rel sasA sasB triple mutant for branched chain amino acids, methionine and threonine PubMed
The protein
Catalyzed reaction/ biological activity
Inosine 5'-phosphate + NAD+ + H2O --> xanthosine 5'-phosphate + NADH (according to UniProt)
Protein family
IMPDH/GMPR family (together with GuaC) (according to UniProt)
Domains
2 CBS domains (aa 95-153, aa 157-214) (according to UniProt)
Structure
3TSB (PDB) (from B. anthracis, 80% identity) PubMed
7OJ1 (PDB) (in complex with Ap4A) PubMed
P21879 (AlphaFold)
Modification
phosphorylated (STY) PubMed
Cys308 is S-cysteinylated after diamide stress PubMed, PubMed
Cys308 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed
Effectors of protein activity
inhibition of enzymatic activity by (p)ppGpp during the stringent response PubMed
binding of Ap4A to the CBS domains results in octamer formation and inhibition of GuaB PubMed
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
guaB
Description
PubMed
Regulation
activated during growth in the presence of branched chain amino acids (CodY) PubMed
Regulatory mechanism
CodY: activation, PubMed, in codY regulon
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guaB

2025-04-05 10:37:13

ghost

103

398e2dfc24deb3b1938cd3d6d5a66996744cbdd0

4BEB5E55C21C03816DFE38C39F868BAC8E73C216

Biological materials
Mutant
BKE00090 (guaA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTAGTAAATCCCCCTCTT, downstream forward: _UP4_TAATAAATTGTTACAAATTA
BKK00090 (guaA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTAGTAAATCCCCCTCTT, downstream forward: _UP4_TAATAAATTGTTACAAATTA
Expression vectors
purification from B. subtilis with an N-terminal Strep-tag, for SPINE, (in pGP380): pGP901, available in Jrg Stlke's lab
pGP2937 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jrg Stlke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jrg Stlke's lab
References
Reviews
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Original Publications
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AB3D18228DB6819B0C81BC7A8BB3408A4F75DC0C

Page visits: 10101

Time of last update: 2025-04-06 04:50:24

Author of last update: Jstuelk