mifM

mifM
168

ribosome-nascent chain sensor of membrane protein biogenesis

Locus
BSU_23880
Molecular weight
11.01 kDa
Isoelectric point
5.83
Protein length
95 aaSequenceBlast
Gene length
288 bpSequenceBlast
Function
control of YidC2 translation
Product
sensor of SpoIIIJ activity
Essential
no
Synonyms
mifM, yqzJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene
Coordinates
2,483,586 → 2,483,873
The protein
Catalyzed reaction/ biological activity
acts as a kind of "leader peptide" that does or does not allow translation through a hairpin structure in the ''yidC2'' mRNA PubMed
Domains
N-terminal transmembrane domain PubMed
the C-terminal domain interacts with the ribosome and arrests the own translation elongation PubMed
Structure
3J9W (PDB) (MifM-stalled ribosome complex) PubMed
EMD-6306 (MifM-stalled ribosome complex) PubMed
Q7WY64 (AlphaFold)
Localization
membrane PubMed
Expression and Regulation
Operons
Genes
mifM-yidC2
Description
PubMed
Regulation
repressed by large amounts of SpoIIIJ, induced by small amounts of SpoIIIJ (MifM) PubMed
Regulatory mechanism
MifM: attenuation, an mRNA hairpin of the yqjG transcript unfold upon translation arrest of MifM at decreased YidC1 levels, in mifM regulon
Additional information
An antisense RNA is predicted for 'mifM' PubMed
Open in new tab

mifM→yidC2

2025-04-16 05:46:07

ghost

114

f8a6fff082045f34969fcbb9e7dd06a79ebdd148

CF4B91E771854E675A6B5052E8B77F0D2D86EFB9

Biological materials
Mutant
BKE23880 (ΔmifM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACATCCATTCAATAGC,  downstream forward: _UP4_TAAACCGCATTTATAAAAAG
BKK23880 (ΔmifM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACATCCATTCAATAGC,  downstream forward: _UP4_TAAACCGCATTTATAAAAAG
References
Loading

C50AE06E8C959EB7176504C8E2BAD1A08E47C51A

Page visits: 7324

Time of last update: 2025-04-22 17:44:17

Author of last update: Jstuelk