khpA

khpA
168

putative RNA chaperone

Locus
BSU_16000
Molecular weight
8.93 kDa
Isoelectric point
7.34
Protein length
Gene length
Function
unknown
Product
putative RNA chaperone
Essential
no
E.C.
null
Synonyms
ylqC, KhpA

Genomic Context

List of homologs in different organisms, belongs to COG1837 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, Stringent response

Gene
Coordinates
1,673,892  1,674,137
The protein
Protein family
UPF0109 family (single member, according to UniProt)
KH domain (aa 34-81) (according to UniProt)
Structure
Expression and Regulation
Operons
Description
Regulation
the mRNA is processed between ffh and rpsP by RNase Y, this requires the RicA-RicF-RicT complex PubMed
Expression
expression is decreased upon depletion of tRNA maturation factors rnpB or rnz PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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Array

2025-01-14 17:28:37

Jstuelk

60

0C22E9A74563E6DB840352CF68BC5D70BBD11EAB

0C22E9A74563E6DB840352CF68BC5D70BBD11EAB

Description
Regulation
the mRNA is processed between ffh and rpsP by RNase Y, this requires the RicA-RicF-RicT complex PubMed
Expression
expression is decreased upon depletion of tRNA maturation factors rnpB or rnz PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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ylxMrplS

2025-01-14 16:59:31

Jstuelk

117

4F2FF9A3B937EE85312D0B0F9DA494E4B44E2297

417C7007C2492335257F8AEF606BB8627753757F

Genes
Description
Regulation
Rel dependent downregulation (Class I) during stringent response PubMed
expression is reduced in motile cells as compared to non-motile cells PubMed
Regulatory mechanism
stringent response: negative regulation, in stringent response
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rpsPkhpA

2025-01-16 03:25:13

Jstuelk

95

EBA4231EDC32B79E8B88019E02F3880BAF1378CE

5C4CCA15D9411D61A7B6109A29C1A8986F928694

Description
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rpsPrplS

2025-01-14 15:58:19

Jstuelk

58

B00427AA69EF576CAC3B8B5C44347F99001CAF0D

B00427AA69EF576CAC3B8B5C44347F99001CAF0D

Description
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rpsPrimM

2025-01-15 18:31:19

Jstuelk

56

B1D4FB4E8DDCF0CB0481B85ABEADC1254ACBB0DF

B1D4FB4E8DDCF0CB0481B85ABEADC1254ACBB0DF

Biological materials
Mutant
MGNA-B173 (ylqC::erm), available at the NBRP B. subtilis, Japan
1A827 (ylqC::erm), PubMed, available at BGSC
BKE16000 (khpA::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_ATCTTCCAAGTGTTGATCAG,  downstream forward: _UP4_TAAAAGGGAGAGGGCCGGCA
BKK16000 (khpA::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_ATCTTCCAAGTGTTGATCAG,  downstream forward: _UP4_TAAAAGGGAGAGGGCCGGCA
Expression vectors
pGP3616 expression of khpA-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
Reviews
Brantl S, Ul Haq ISmall proteins in Gram-positive bacteria.FEMS microbiology reviews. 2023 Nov 1; 47(6). PMID: 38052429
Wicke D, Meißner J, Warneke R, Elfmann C, Stülke JUnderstudied proteins and understudied functions in the model bacterium Bacillus subtilis-A major challenge in current research.Molecular microbiology. 2023 Mar 7; . PMID: 36882621
Olejniczak M, Jiang X, Basczok MM, Storz GKH-domain proteins: another family of bacterial RNA matchmakers?Molecular microbiology. 2021 Nov 8; . PMID: 34748246
References
Trinquier A, Condon C, Braun FEffect of tRNA Maturase Depletion on Levels and Stabilities of Ribosome Assembly Cofactor and Other mRNAs in Bacillus subtilis.Microbiology spectrum. 2023 Feb 22; :e0513422. PMID: 36840557
Honda K, Nakamura K, Nishiguchi M, Yamane K Cloning and characterization of a Bacillus subtilis gene encoding a homolog of the 54-kilodalton subunit of mammalian signal recognition particle and Escherichia coli Ffh. Journal of bacteriology. 1993 Aug; 175(15):4885-94. . PMID:8335643

67FB5F6FB0B2701BD8E4FDD8DF06B236DE6A2599

Page visits: 2655

Time of last update: 2025-01-17 17:12:13

Author of last update: Jstuelk