fusA

fusA
168

elongation factor G, catalyzes translocation steps of the ribosome after peptide bond formation, facilitates movement of tRNA and mRNA by one codon

Locus
BSU_01120
Molecular weight
76.36 kDa
Isoelectric point
4.61
Protein length
Gene length
Function
translation
Product
elongation factor G
Essential
yes
Synonyms
fusA, fus

Genomic Context

List of homologs in different organisms, belongs to COG0480 (Galperin et al., 2021)

This gene is a member of the following regulons

Stringent response

Gene
Coordinates
130,684 132,762
Phenotypes of a mutant
essential PubMed
specific point mutations affecting FusA facilitate the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate PubMed
The protein
Catalyzed reaction/ biological activity
hydrolyses GTP
catalyzes translocation steps of the ribosome after peptide bond formation, facilitates movement of tRNA and mRNA by one codon PubMed
Protein family
tr-type G domain (aa 8-282) (according to UniProt)
Structure
2XEX (PDB) (from Staphylococcus aureus, 78.35% identity) PubMed
1ELO (PDB) (from Thermus thermophilus) PubMed
4BTC (PDB), 4BTD (PDB), the Thermus thermophilus EF-Gribosome complex in a pretranslocation state PubMed
Modification
phosphorylation on Ser-213 AND Ser-302 AND Ser-569 AND Ser-680 AND (Thr-24 OR Thr-25) AND (Thr-43 OR Ser 48) PubMed, PubMed
phosphorylated on Tyr-339 PubMed
cytoplasm (according to UniProt)
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Description
Regulation
Rel dependent downregulation (Class I) during stringent response PubMed
the mRNA is processed between rplGB and rpsL by RNase Y, this requires the RicA-RicF-RicT complex PubMed
Regulatory mechanism
stringent response: negative regulation, PubMed, in stringent response
Open in new tab

rplGB->tufA

2025-01-11 05:35:36

Jstuelk

107

d846304cc2bd15e30cde627ac53b7d142c24fbb5

614BD7852C60161107389373FB106F44BAE4C835

Biological materials
Expression vectors
for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP840, available in Jrg Stlke's lab
for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP848, available in Jrg Stlke's lab
References
Reviews
Agirrezabala X, Frank JElongation in translation as a dynamic interaction among the ribosome, tRNA, and elongation factors EF-G and EF-Tu.Quarterly reviews of biophysics. 2009 Aug; 42(3):159-200. PMID: 20025795
Original Publications
Demo G, Gamper HB, Loveland AB, Masuda I, Carbone CE, Svidritskiy E, Hou YM, Korostelev AAStructural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation.Nature communications. 2021 Jul 30; 12(1):4644. PMID: 34330903
Krüger L, Herzberg C, Rath H, Pedreira T, Ischebeck T, Poehlein A, Gundlach J, Daniel R, Völker U, Mäder U, Stülke JEssentiality of c-di-AMP in Bacillus subtilis: Bypassing mutations converge in potassium and glutamate homeostasis.PLoS genetics. 2021 Jan 22; 17(1):e1009092. PMID: 33481774
Sharma H, Adio S, Senyushkina T, Belardinelli R, Peske F, Rodnina MV Kinetics of Spontaneous and EF-G-Accelerated Rotation of Ribosomal Subunits. Cell reports. 2016 Aug 23; 16(8):2187-96. doi:10.1016/j.celrep.2016.07.051. pii:S2211-1247(16)30982-2. PMID:27524615
Rosenberg A, Soufi B, Ravikumar V, Soares NC, Krug K, Smith Y, Macek B, Ben-Yehuda S Phosphoproteome dynamics mediate revival of bacterial spores. BMC biology. 2015 Sep 17; 13:76. doi:10.1186/s12915-015-0184-7. PMID:26381121
Adio S, Senyushkina T, Peske F, Fischer N, Wintermeyer W, Rodnina MV Fluctuations between multiple EF-G-induced chimeric tRNA states during translocation on the ribosome. Nature communications. 2015 Jun 15; 6:7442. doi:10.1038/ncomms8442. PMID:26072700
Liu G, Song G, Zhang D, Zhang D, Li Z, Lyu Z, Dong J, Achenbach J, Gong W, Zhao XS, Nierhaus KH, Qin Y EF-G catalyzes tRNA translocation by disrupting interactions between decoding center and codon-anticodon duplex. Nature structural & molecular biology. 2014 Sep; 21(9):817-24. doi:10.1038/nsmb.2869. PMID:25108354
Holtkamp W, Cunha CE, Peske F, Konevega AL, Wintermeyer W, Rodnina MV GTP hydrolysis by EF-G synchronizes tRNA movement on small and large ribosomal subunits. The EMBO journal. 2014 May 02; 33(9):1073-85. doi:10.1002/embj.201387465. PMID:24614227
Chen Y, Feng S, Kumar V, Ero R, Gao YG Structure of EF-G-ribosome complex in a pretranslocation state. Nature structural & molecular biology. 2013 Sep; 20(9):1077-84. doi:10.1038/nsmb.2645. PMID:23912278
. . PMID: 20718859
Clementi N, Chirkova A, Puffer B, Micura R, Polacek N Atomic mutagenesis reveals A2660 of 23S ribosomal RNA as key to EF-G GTPase activation. Nature chemical biology. 2010 May; 6(5):344-51. doi:10.1038/nchembio.341. PMID:20348921
Eymann C, Becher D, Bernhardt J, Gronau K, Klutzny A, Hecker M Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis. Proteomics. 2007 Oct; 7(19):3509-26. . PMID:17726680
Macek B, Mijakovic I, Olsen JV, Gnad F, Kumar C, Jensen PR, Mann M The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis. Molecular & cellular proteomics : MCP. 2007 Apr; 6(4):697-707. . PMID:17218307
Eymann C, Dreisbach A, Albrecht D, Bernhardt J, Becher D, Gentner S, Tam le T, Büttner K, Buurman G, Scharf C, Venz S, Völker U, Hecker M A comprehensive proteome map of growing Bacillus subtilis cells. Proteomics. 2004 Oct; 4(10):2849-76. . PMID:15378759
AEvarsson A, Brazhnikov E, Garber M, Zheltonosova J, Chirgadze Y, al-Karadaghi S, Svensson LA, Liljas A Three-dimensional structure of the ribosomal translocase: elongation factor G from Thermus thermophilus. The EMBO journal. 1994 Aug 15; 13(16):3669-77. . PMID:8070397

297844645CCCF40CB40E84339997F6610883DF4E

Page visits: 6160

Time of last update: 2025-01-17 14:03:47

Author of last update: Jstuelk