tsaC
168

L-threonylcarbamoyl-AMP synthase, biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A) (together with TsaB-TsaD-TsaE)

Locus

BSU_36950

Molecular weight

36.85 kDa

Isoelectric point

5.82

Protein length

346 aaSequenceBlast

Gene length

1041 bpSequenceBlast

Function

tRNA modification

Product

L-threonylcarbamoyl-AMP synthase

Essential

E.C.

2.7.7.87

Synonyms

tsaC, ipc-29d, ywlC

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0009 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

3,792,969 → 3,794,009

Phenotypes of a mutant

poor growth PubMed

poorly transformable PubMed

restores viability of a ''smc'' mutant on rich medium due to the induction of a stringent response PubMed

The protein

Catalyzed reaction/ biological activity

conversion of L-threonine, bicarbonate/CO2 and ATP to give the intermediate L-threonylcarbamoyl-AMP (TC-AMP) and pyrophosphate as products PubMed

biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A), a universal modification found at position 37 of ANN decoding tRNAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vitro (shown for the corresponding E. coli protein YrdC) PubMed

ATP + hydrogencarbonate + L-threonine --> diphosphate + H₂O + L-threonylcarbamoyladenylate (according to UniProt)

Protein family

SUA5 family (single member, according to UniProt)

Domains

YrdC-like domains (aa 18-205) (according to UniProt)

Structure

1HRU (PDB) (the homolog from E. coli) PubMed

P39153 (AlphaFold)

Expression and Regulation

Operons

Genes

tsaC