iolA

iolA
168

methylmalonate-semialdehyde dehydrogenase (acylating)

Locus
BSU_39760
Molecular weight
53.29 kDa
Isoelectric point
5.14
Protein length
Gene length
Function
myo-inositol catabolism
Product
methylmalonate-semialdehyde dehydrogenase (acylating)
Essential
no
E.C.
1.2.1.27
Synonyms
iolA, mmsA, yxdA

Genomic Context

List of homologs in different organisms, belongs to COG1012 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,082,920  4,084,383
The protein
Catalyzed reaction/ biological activity
NAD-dependent oxidation of methylmalonate semialdehyde (MMSA) to propionyl-CoA via acylation and deacylation steps
3-oxopropanoate + CoA + NAD+ --> acetyl-CoA + CO2 + NADH (according to UniProt)
2-methyl-3-oxopropanoate + CoA + H2O + NAD+ --> H+ + hydrogencarbonate + NADH + propanoyl-CoA (according to UniProt)
Protein family
aldehyde dehydrogenase family (according to UniProt)
Coenzyme A, NAD PubMed
Structure
Paralogous protein(s)
Expression and Regulation
Operons
Description
Regulation
induced by inositol (IolR) PubMed
Regulatory mechanism
IolR: repression, PubMed, in iolR regulon
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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iolAiolJ

2025-01-11 21:13:47

ghost

110

4fd092965338ef1511600132442d2247a2693c98

C19E63046F0B412961D2626F72E0308D922D50B2

Biological materials
Mutant
MGNA-B770 (iolA::erm), available at the NBRP B. subtilis, Japan
BKE39760 (iolA::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTCTTATTGCCTCCTTCA,  downstream forward: _UP4_TAAACGACGAACAGCCGAAC
BKK39760 (iolA::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTCTTATTGCCTCCTTCA,  downstream forward: _UP4_TAAACGACGAACAGCCGAAC
Labs working on this gene/protein
Yasutaro Fujita, University of Fukuyama, Japan
[[Ken-ichi Yoshida]], Kobe University, Japan
References
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762718A15E5256261D79DF60F9106AF0CE2D60C6

Page visits: 5143

Time of last update: 2025-01-18 15:05:59

Author of last update: Melvin.boenninger