recJ

recJ
168

single-strand DNA-specific exonuclease, acts together with RecQ in replication fork maintenance, crucial for the RecA-dependent acquisition of homologous genes from related species by natural transformation

Locus
BSU_27620
Molecular weight
87.83 kDa
Isoelectric point
5.18
Protein length
786 aaSequenceBlast
Gene length
2361 bpSequenceBlast
Function
replication fork maintenance
Product
single-strand DNA-specific exonuclease
Essential
no
Synonyms
recJ, yrvE
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4199 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,823,419 → 2,825,779
Phenotypes of a mutant
a recU recJ double mutant is not viable PubMed
''recO recJ'' double mutants are extremely sensitive against DNA damaging agents PubMed
suppression of lethality of pcrA inactivation PubMed
improved survival after DNA replication arrest imposed by inhibition of PolC activity PubMed
The protein
Protein family
RecJ family (with YorK, according to UniProt)
Domains
DHH-DHHA1 domain
Cofactors
Mn2+ PubMed
Structure
5F54 (PDB) (from Deinococcus radiodurans, the DHH-DHHA1 domain, 32% identity) PubMed
O32044 (AlphaFold)
Paralogous protein(s)
YorK
Localization
associated with the replisome PubMed
bound to replication forks, but also assembles at many sites on the nucleoid upon DNA damage induction PubMed
Expression and Regulation
Operons
Genes
recJ-apt
Description
PubMed
Regulation
Rel dependent downregulation (Class I) during stringent response PubMed
Regulatory mechanism
stringent response: negative regulation, in stringent response
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recJ→apt

2025-04-04 07:23:36

Jstuelk

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B255582EBC06D6885BA9D186E0A7757EA36D195A

Additional information
translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
Biological materials
Mutant
GP895 (recJ::kan), available in Jörg Stülke's lab PubMed
MGNA-B516 (yrvE::erm), available at the NBRP B. subtilis, Japan
BKE27620 (''recJ::erm'', available in the BGSC, in Fabian Commichau's, and in Jörg Stülke's labs) PubMed
BKE27620 (ΔrecJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAATTCACCCCTCAACC,  downstream forward: _UP4_AGTACGAGGAGGACATAAAA
BKK27620 (ΔrecJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAATTCACCCCTCAACC,  downstream forward: _UP4_AGTACGAGGAGGACATAAAA
References
Reviews
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Original Publications
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BA755FA1CB1E0C006E9A23489A7C8997141AA498

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Time of last update: 2025-04-12 16:08:29

Author of last update: Jstuelk