ansB

ansB
168

L-aspartase

Locus
BSU_23570
Molecular weight
52.39 kDa
Isoelectric point
5.69
Protein length
475 aaSequenceBlast
Gene length
1428 bpSequenceBlast
Function
aspartate degradation
Product
L-aspartase
Essential
no
E.C.
4.3.1.1
Synonyms
ansB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1027 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,454,347  2,455,774
The protein
Catalyzed reaction/ biological activity
L-aspartate --> fumarate + NH4+ (according to UniProt)
Protein family
class-II fumarase/aspartase family (with CitG, according to UniProt)
Structure
3R6Q (PDB) (from Bacillus sp. YM55-1, 72% identity, 92% similarity) PubMed
P26899 (AlphaFold)
Paralogous protein(s)
CitG
Expression and Regulation
Operons
Genes
ansA-ansB
Description
PubMed
Regulation
expressed in the presence of asparagine (AnsR) PubMed
expression is reduced in motile cells as compared to non-motile cells PubMed
Regulatory mechanism
AnsR: repression, PubMed, in ansR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

ansA→ansB

2025-04-04 08:39:03

Jstuelk

89

26B0E8E9928B9258DD0E99A3A3CCC1B9E0B8F068

0371500E4D8DAAD3D8BE963EF8C39327F0863D20

Genes
ansA-ansB-mleN-mleA
Description
PubMed
Regulation
AnsA: expressed in the presence of asparagine (AnsR) PubMed
Expression
expressed in the presence of asparagine (AnsR) PubMed
expression is reduced in motile cells as compared to non-motile cells PubMed
Regulatory mechanism
AnsR: transcription repression, PubMed, in ansR regulon
Open in new tab

ansA→mleA

2025-04-04 08:53:37

Jstuelk

102

6E6CC6ADE2F20EBE93C9F1ED4C2EF4E87AF126B1

D7F0E184034763E326DE2841E35068D2FD73DD5D

Biological materials
Mutant
GP1153 (ΔansA-ansB::ermC) available in Jörg Stülke's lab
GP4256 (ΔansA-ansB::cat) available in Jörg Stülke's lab
BKE23570 (ansB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATCTTTAACCTTCTTT,  downstream forward: _UP4_TAAAATTTGTATAAAATACA
BKK23570 (ansB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATCTTTAACCTTCTTT,  downstream forward: _UP4_TAAAATTTGTATAAAATACA
LacZ fusion
pGP2274 (in pAC5) (GP2961), available in Jörg Stülke's lab
References
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5FAE80F22AEBAE63FC3C872EC651166A65C34DC2

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Time of last update: 2025-04-13 03:47:52

Author of last update: Jstuelk