Difference between revisions of "SubtInteract"

From SubtiWiki
Jump to: navigation, search
(→‎Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis))
 
(39 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as [[DNA replication]], [[transcription]] or [[translation]]. Protein-protein interactions may also be involved in a variety of regulatory events. Finally, many interactions may be of a transient nature.
+
Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as [[DNA replication]], [[transcription]] or [[translation]]. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.
  
−
==Methods to detect protein-protein interactions==
+
<br/>
−
* Bacterial Two Hybrid System: [[BACTH]]
+
<big>'''Now online: A description of ''Subti''Wiki, ''Subti''Pathways, and ''Subt''Interact in the 2012 Database issue of Nucleic Acids Research'''</big>
 +
<pubmed> 22096228 </pubmed>
 +
 
 +
=Methods to detect protein-protein interactions=
 +
* Bacterial Two Hybrid System: [[BACTH]] {{PubMed|9576956}}
  
 
* Yeast Two Hybrid System {{PubMed|18218650,16806892,16262681,15064463,11769011,10967325,8816737,7894083}}
 
* Yeast Two Hybrid System {{PubMed|18218650,16806892,16262681,15064463,11769011,10967325,8816737,7894083}}
  
−
* [[SPINE]]: A method to detect ''in vivo'' protein-protein interactions after cross-linking
+
* [[SPINE]]: A method to detect ''in vivo'' protein-protein interactions after cross-linking {{PubMed|17994626}}
  
 
* TAP-Tag purification {{PubMed|10504710,11403571}}  
 
* TAP-Tag purification {{PubMed|10504710,11403571}}  
Line 12: Line 16:
 
* ''in vivo'' detection of protein-protein interaction using [[DivIVA]] and GFP {{PubMed|19698693}}
 
* ''in vivo'' detection of protein-protein interaction using [[DivIVA]] and GFP {{PubMed|19698693}}
  
−
==Important publications==
+
'''Attention:''' Each technique detects only about 33% of all interactions {{PubMed|20805792,19060903}}
−
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303  </pubmed>
+
 
 +
=Visualization of protein-protein interactions in ''B. subtilis''=
 +
* [http://cellpublisher.gobics.de/subtinteract/startpage/start/ interactive protein-protein interaction map]
 +
* the beta version of [http://subtiwiki.uni-goettingen.de/pathways/SubtInteract/demo_mitlinks/ SubtInteract]
 +
 
 +
=Protein complexes in ''B. subtilis''=
 +
==Complexes in [[Cellular processes]]==
 +
=== [[cell wall synthesis]] and [[cell shape]]: the [[elongasome]]===
 +
=== [[cell division]]: the [[divisome]]===
 +
 
 +
== Complexes in [[metabolism]], [[ATP synthesis]] and [[respiration]] ==
 +
=== the metabolons of glycolysis and the TCA cycle {{PubMed|19193632,20933603}}===
 +
=== the [[ATP synthase]] ===
 +
=== [[respiratory complexes and supercomplexes]] ===
 +
 
 +
==Complexes in [[Information processing]]==
 +
=== Initiation of [[DNA replication]]: the [[primosome]]===
 +
=== [[DNA replication]]: the [[replisome]]===
 +
=== origin segregation: [[condensin]] ===
 +
=== [[transcription]]: [[RNA polymerase]]===
 +
=== [[translation]]: the [[ribosomal proteins|ribosome]]===
 +
=== synthesis of glutamyl-tRNA(Gln): the [[transamidosome]] ([[GatA]]-[[GatB]]-[[GatC]])-[[GltX]]-[[trnS-Gln]]===
 +
=== [[RNases|RNA processing and degradation]]: the [[RNA degradosome]]===
 +
=== [[protein secretion]]: the [[signal recognition particle]] ===
 +
 
 +
==Complexes involved in [[Lifestyles]]==
 +
=== [[General stress proteins (controlled by SigB)|general stress response]]: the [[stressosome]]===
 +
=== DNA uptake: the [[pseudopilus]] {{PubMed|21278288,16751195}}===
 +
===spore [[germination]]: the [[germinosome]]===
 +
 
 +
=Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis)=
 +
* [[FruA]], [[SwrC]], [[XhlA]], [[YhaP]], [[YhgE]], [[YkcC]], [[PgpH]], [[CsbC]], [[CssS]], [[FliZ]], [[MreD]], [[PpsC]], [[RacA]], [[Smc]], [[YclI]], [[YdeL]], [[YdgH]], [[YdbI]], [[RhgR]], [[YkoT]], [[YopZ]], [[YqbD]], [[RmgR]], [[YueB]], [[YwqJ]], [[YyxA]]
 +
<pubmed> 21630458</pubmed>
 +
 
 +
=Important publications=
 +
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 18366733 10694888 2200717 2441660 20969605 15451506 9787636 22790590 23808343 </pubmed>

Latest revision as of 13:48, 8 May 2015

Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.


Now online: A description of SubtiWiki, SubtiPathways, and SubtInteract in the 2012 Database issue of Nucleic Acids Research

Ulrike Mäder, Arne G Schmeisky, Lope A Flórez, Jörg Stülke
SubtiWiki--a comprehensive community resource for the model organism Bacillus subtilis.
Nucleic Acids Res: 2012, 40(Database issue);D1278-87
[PubMed:22096228] [WorldCat.org] [DOI] (I p)


Methods to detect protein-protein interactions

  • Yeast Two Hybrid System PubMed
  • SPINE: A method to detect in vivo protein-protein interactions after cross-linking PubMed
  • in vivo detection of protein-protein interaction using DivIVA and GFP PubMed

Attention: Each technique detects only about 33% of all interactions PubMed

Visualization of protein-protein interactions in B. subtilis

Protein complexes in B. subtilis

Complexes in Cellular processes

cell wall synthesis and cell shape: the elongasome

cell division: the divisome

Complexes in metabolism, ATP synthesis and respiration

the metabolons of glycolysis and the TCA cycle PubMed

the ATP synthase

respiratory complexes and supercomplexes

Complexes in Information processing

Initiation of DNA replication: the primosome

DNA replication: the replisome

origin segregation: condensin

transcription: RNA polymerase

translation: the ribosome

synthesis of glutamyl-tRNA(Gln): the transamidosome (GatA-GatB-GatC)-GltX-trnS-Gln

RNA processing and degradation: the RNA degradosome

protein secretion: the signal recognition particle

Complexes involved in Lifestyles

general stress response: the stressosome

DNA uptake: the pseudopilus PubMed

spore germination: the germinosome

Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis)


Important publications

Retrieved from "http://subtiwiki.uni-goettingen.de/wiki//index.php?title=SubtInteract&oldid=207886"