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− | * '''Description:''' amino acid racemase, production of D-amino acids, control of [[biofilm formation]] <br/><br/>
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− | {| align="right" border="1" cellpadding="2"
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− | |style="background:#ABCDEF;" align="center"|'''Gene name'''
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− | |''racX''
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− | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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− | |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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− | |style="background:#ABCDEF;" align="center"| '''Product''' || amino acid racemase
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− | |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[biofilm formation]]
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− | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 5.396
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− | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 681 bp, 227 aa
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− | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yveF]]'', ''[[pbpE]]''
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− | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15448]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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− | |colspan="2" | '''Genetic context''' <br/> [[Image:racX_context.gif]]
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− | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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− | |}
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− | __TOC__
| + | [[File:Example.jpg]] |
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− | <br/><br/>
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− | = [[Categories]] containing this gene/protein =
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− | {{SubtiWiki category|[[biofilm formation]]}},
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− | {{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, W, X, Y)]]}}
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− | = This gene is a member of the following [[regulons]] =
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− | {{SubtiWiki regulon|[[AbrB regulon]]}},
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− | {{SubtiWiki regulon|[[SigW regulon]]}}
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− | =The gene=
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− | === Basic information ===
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− | * '''Locus tag:''' BSU34430
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− | ===Phenotypes of a mutant ===
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− | === Database entries ===
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− | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pbpE-racX.html]
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− | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10391]
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− | === Additional information===
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− | =The protein=
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− | === Basic information/ Evolution ===
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− | * '''Catalyzed reaction/ biological activity:'''
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− | * '''Protein family:''' aspartate/glutamate racemases family (according to Swiss-Prot)
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− | * '''Paralogous protein(s):'''
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− | === Extended information on the protein ===
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− | * '''Kinetic information:'''
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− | * '''Domains:'''
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− | * '''Modification:'''
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− | * '''Cofactor(s):'''
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− | * '''Effectors of protein activity:'''
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− | * '''Interactions:'''
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− | * '''Localization:'''
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− | === Database entries ===
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− | * '''Structure:'''
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− | * '''UniProt:''' [http://www.uniprot.org/uniprot/P32960 P32960]
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− | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU34430]
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− | * '''E.C. number:'''
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− | === Additional information===
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− | =Expression and regulation=
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− | * '''Operon:''' ''[[pbpE]]-[[racX]]'' {{PubMed|8491712}}
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− | * '''[[Sigma factor]]:''' [[SigW]] {{PubMed|8491712,12207695}}
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− | * '''Regulation:'''
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− | ** repressed during logrithmic growth ([[AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/7592498 PubMed]
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− | ** induced by cell wall stress ([[SigW]]) {{PubMed|8491712,12207695}}
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− | * '''Regulatory mechanism:'''
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− | ** [[AbrB]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/7592498 PubMed]
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− | * '''Additional information:'''
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− | =Biological materials =
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− | * '''Mutant:'''
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− | * '''Expression vector:'''
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− |
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− | * '''lacZ fusion:'''
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− | * '''GFP fusion:'''
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− | * '''two-hybrid system:'''
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− | * '''Antibody:'''
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− | =Labs working on this gene/protein=
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− | =Your additional remarks=
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− | =References=
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− | <pubmed>8491712,19063962,9987136, 20431016,7592498, </pubmed>
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− | [[Category:Protein-coding genes]]
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