pucI

pucI
168

allantoin permease

Locus
BSU_36470
Molecular weight
53.82 kDa
Isoelectric point
7.88
Protein length
Gene length
Function
purine utilization
Product
allantoin permease
Essential
no
Synonyms
pucI, ywoE

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1953 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,752,280  3,753,752
The protein
Catalyzed reaction/ biological activity
uptake of allantoin PubMed
Protein family
purine-cytosine permease (2.A.39) family (with YxlA, according to UniProt)
Structure
2JLN (PDB) (from Microbacterium liquefaciens, 27% identity) PubMed
cell membrane PubMed
Expression and Regulation
Operons
Genes
Description
Regulation
expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
Regulatory mechanism
PucR: activation, PubMed, in pucR regulon
Open in new tab

pucI

2025-01-19 14:07:42

ghost

62

35767b4b5880e7c94aa2949e38595330429a12ee

96412BDE76472AECD95CE78CE864D73C344AF751

Biological materials
Mutant
MGNA-A897 (ywoE::erm), available at the NBRP B. subtilis, Japan
BKE36470 (pucI::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATAGCCGGCTCCCCTTTCC,  downstream forward: _UP4_TAAGATCTATTTAGACGGTA
BKK36470 (pucI::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATAGCCGGCTCCCCTTTCC,  downstream forward: _UP4_TAAGATCTATTTAGACGGTA
BP1261 (pucI::cat trp+) available in Jörg Stülke's & Fabian Commichau's lab
References
Loading

5681A5F186AD4B8C81B78FD9DDAE2C0624BE7CAF

Page visits: 5655

Time of last update: 2025-01-27 18:44:59

Author of last update: Melvin.boenninger