spoVAD

spoVAD
168

essential for the uptake of the 1:1 chelate of pyridine-2,6-dicarboxylic acid (DPA(2,6)) and Ca(2 ) into developing spores and DPA release during Germination, likely germination protein

Locus
BSU_23410
Molecular weight
35.84 kDa
Isoelectric point
5.24
Protein length
338 aaSequenceBlast
Gene length
1017 bpSequenceBlast
Function
likely germination protein
Product
binding protein for DPA
Essential
no
Synonyms
spoVAD
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5837 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,440,775  2,441,791
The protein
Catalyzed reaction/ biological activity
maybe involved in spore germination PubMed
binding of both DPA(2,6) and Ca(2 )-DPA(2,6) PubMed
forms a membrane complex with SpoVAC and SpoVAEB in vitro PubMed
functions like a "plug" for the SpoVAC/SpoVAEB membrane channel PubMed
Structure
3LM6 (PDB)
P40869 (AlphaFold)
Localization
cytoplasmic side of the spore inner membrane - that depends on SpoVAC/SpoVAEB PubMed
Additional information
6,500 molecules are present per spore PubMed
Expression and Regulation
Operons
Genes
spoVAA-spoVAB-spoVAC-spoVAD-spoVAEB-spoVAEA-spoVAF-lysA
Description
PubMed
Regulation
expressed late during sporulation in the forespore (SigG, SpoVT) PubMed
Regulatory mechanism
SpoVT: activation, PubMed, in spoVT regulon
Sigma factors
SigG: sigma factor, PubMed, in sigG regulon
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spoVAA→lysA

2025-04-23 18:37:14

Jstuelk

132

12fce0add6fb0abbed0128cd99d7be2c377e96d5

420F73950DA10EFCB3126E84B350E66BA95D34DD

Additional information
6,500 molecules are present per spore PubMed
Biological materials
Mutant
BKE23410 (spoVAD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGTTAATTTCATTTTCTTCT,  downstream forward: _UP4_GAGCGTGCAGGAGGTGCATC
BKK23410 (spoVAD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGTTAATTTCATTTTCTTCT,  downstream forward: _UP4_GAGCGTGCAGGAGGTGCATC
Labs working on this gene/protein
Peter Setlow, University of Connecticut Health Center, USA
References
Reviews
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Original Publications
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9531F0A0DC4AE5696FFBAC9C2A773F15840E3255

Page visits: 3960

Time of last update: 2025-04-23 23:51:28

Author of last update: Jstuelk