epsM

epsM
168

UDP-2,4,6-trideoxy-2-acetamido-4-amino glucose acetyltransferase, extracellular polysaccharide synthesis

Locus
BSU_34240
Molecular weight
22.34 kDa
Isoelectric point
9.43
Protein length
Gene length
Function
Biofilm formation, biosynthesis of UDP-N,N’-diacetylbacillosamine (with EpsC and EpsN)
Product
UDP-2,4,6-trideoxy-2-acetamido-4-amino glucose acetyltransferase
Essential
no
Synonyms
epsM, yvfD

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0110 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,516,233  3,516,883
Phenotypes of a mutant
smooth colonies on MsGG medium, no biofilm formation PubMed
The protein
Catalyzed reaction/ biological activity
UDP-2,4,6-trideoxy-2-acetamido-4-amino glucose + acetyl-CoA --> UDP-2,4,6-trideoxy-2,4-diacetamido glucose (UDP-N,N'-diacetylbacillosamine) + CoA PubMed
Protein family
coenzyme A PubMed
Structure
4M99 (PDB) (from Neisseria gonorrhoeae, 38% identity) PubMed
Expression and Regulation
Operons
Description
Regulation
repressed by SinR PubMed
Regulatory mechanism
RemA: activation, PubMed, in remA regulon
AbrB: repression, PubMed, in abrB regulon
SinR: anti-activation, (prevents binding of RemA) PubMed, in sinR regulon
EAR riboswitch: processive antitermination, in EAR riboswitch
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
induction by sequestration of SinR by SinI or SlrA PubMed
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epsAepsO

2025-01-24 04:29:01

ghost

143

972f8e32dbf02ccc05ec1b86b52f3e7c715bf4da

DAC70A15EA1F43CB18727990D26DF72F59759165

Biological materials
Mutant
MGNA-B609 (yvfD::erm), available at the NBRP B. subtilis, Japan
BKE34240 (epsM::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_GTCACCCACAATGGCCACAT,  downstream forward: _UP4_CAAACATCAAACAAAGGATG
BKK34240 (epsM::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_GTCACCCACAATGGCCACAT,  downstream forward: _UP4_CAAACATCAAACAAAGGATG
Labs working on this gene/protein
Richard Losick, Harvard Univ., Cambridge, USA homepage
References
Reviews
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Research papers
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The EAR RNA switch
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40EA584CF77FFF7D7E4E74B414EC69ADE3A1585B

Page visits: 3941

Time of last update: 2025-01-24 18:43:35

Author of last update: Jstuelk