ptsG
168
glucose permease of the phosphotransferase system, EIICBA of the PTS, Trigger enzyme, control of GlcT activity
Locus
BSU_13890
Molecular weight
75.34 kDa
Isoelectric point
5.41
Product
glucose permease, trigger enzyme
Essential
no
E.C.
2.7.1.69
Synonyms
ptsG, ptsX, crr
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2190 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,457,187 → 1,459,286
The protein
Catalyzed reaction/ biological activity
transport and phosphorylation of glucose, receives a phosphate from HPr]] at the IIA domain (His-620), the phosphate group is then transferred to the IIB domain (Cys-461) an finally to the incoming glucose. In the absence of glucose, PtsG phosphorylates and thereby inactivates the transcriptional antiterminator GlcT.
D-glucose + Nπ-phospho-L-histidyl-[protein] --> D-glucose-6-P + L-histidyl-[protein] (according to UniProt)
Protein family
11x transmembrane domain (16–36, 89–109, 139–159, 180–200, 233–253, 283–303, 313–333, 338–358, 365–385, 388–408)
PTS EIIA domain type-1 (aa 568–672) (according to UniProt)
PTS EIIB domain type-1 (aa 439–520) (according to UniProt)
PTS EIIC domain type-1 (aa 1-424) (according to UniProt)
Structure
8QSR (PDB) (the IIC dimer of E. coli PtsG, 47% identity) PubMed
5IWS (PDB) (the IIC domain of B. cereus MalT, 32% identity) PubMed
1AX3 (PDB) (IIA domain) PubMed
1GPR (PDB) (IIA domain)
Modification
transient phosphorylation (HPr]]-dependent) on His-620, then internal phosphotransfer from His-620 to Cys-461
Paralogous protein(s)
membrane protein PubMed
Expression and Regulation
Operons
Description
Regulation
Regulatory mechanism
stringent response: negative regulation, in stringent response
Sigma factors
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Biological materials
Mutant
Expression vectors
pGP123 (domains BA, in pWH844), available in Jörg Stülke's lab
pGP141 (domains BA, mut: H620D, in pWH844), available in Jörg Stülke's lab
pGP428 (EIIB, in pWH844), available in Jörg Stülke's lab
pGP437(EIIA in pGP570, with thrombin cleavage site), available in Jörg Stülke's lab
LacZ fusion
pGP606 (mutant terminator, pAC6), available in Jörg Stülke's lab
pGP532 (pAC7), available in Jörg Stülke's lab
series of RAT mutants are available in pAC6, available in Jörg Stülke's lab
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany Homepage
References
Reviews
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Original Publications
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Time of last update: 2025-01-24 19:52:57
Author of last update: Jstuelk