ldh

ldh
168

L-lactate dehydrogenase

Locus
BSU_03050
Molecular weight
34.00 kDa
Isoelectric point
5.394
Protein length
321 aaSequenceBlast
Gene length
966 bpSequenceBlast
Function
overflow metabolism, fermentation
Product
L-lactate dehydrogenase
Essential
no
E.C.
1.1.1.27
Synonyms
ldh, lctE
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0039 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
329,774  330,739
The protein
Catalyzed reaction/ biological activity
(S)-lactate + NAD+ --> H+ + NADH + pyruvate (according to UniProt)
Protein family
LDH/MDH superfamily (with Mdh, according to UniProt)
Structure
3PQE (PDB)
A general discussion of Ldh structure (PDB)
P13714 (AlphaFold)
Modification
phosphorylation on Tyr-224 PubMed
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
ldh-lctP
Description
PubMed
Regulation
induced under anaerobic conditions (Rex) PubMed
Regulatory mechanism
Rex: repression, PubMed, in rex regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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ldh→lctP

2025-04-03 13:36:27

ghost

102

4e780837233d2e8aaeda4442db171cadf439e394

20AF87A98A20E424287B9BFAEFDE8827032D1500

Biological materials
Mutant
BKE03050 (ldh::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAATCATCCTTCCAGGG,  downstream forward: _UP4_TAACCGCAACTTTAGAGTAA
BKK03050 (ldh::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAATCATCCTTCCAGGG,  downstream forward: _UP4_TAACCGCAACTTTAGAGTAA
GP2597 (ldh::tet comIQ12L) (in DK1042) available in Jörg Stülke's lab
GP4210 (ldh::tet), available in Jörg Stülke's lab
LacZ fusion
pGP3326 (cat, based on pAC5]), available in Jörg Stülke's lab
References
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A0DD68FE90FD13DF03496321AB2DAAADF9F4230D

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Time of last update: 2025-04-09 12:19:44

Author of last update: Christoph.elfmann