dusB2

dusB2
168

minor tRNA-dihydrouridine synthase 2

Locus
BSU_08030
Molecular weight
36.83 kDa
Isoelectric point
5.38
Protein length
Gene length
Function
tRNA modification
Product
tRNA-dihydrouridine synthase 2
Essential
no
Synonyms
yfjN, dusC, dus2

Genomic Context

List of homologs in different organisms, belongs to COG0042 (Galperin et al., 2021)

Gene
Coordinates
876,426 → 877,403
The protein
Catalyzed reaction/ biological activity
5,6-dihydrouridine in tRNA + NAD+ → uridine in tRNA + H+ + NADH PubMed
5,6-dihydrouridine in tRNA + NADP+ → uridine in tRNA + H+ + NADPH PubMed
Protein family
Dus family (with DusB1, according to UniProt)
FMN PubMed
Structure
3B0P (PDB) (from Thermus thermophilus, 24% identity, from aa 12 ... 263) PubMed
Paralogous protein(s)
Expression and Regulation
Operons
Genes
Description
Regulation
the leader mRNA is processed by RNase Y, this requires the RicA-RicF-RicT complex PubMed
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dusB2

2025-01-27 01:36:07

Jstuelk

143

533af6490ff87959a5b9e04f2a6ce1d0eeee86a7

90C3E4122ED23B7B59E83D63D26180C99D78E210

Biological materials
Mutant
MGNA-C277 (yfjN::erm), available at the NBRP B. subtilis, Japan
BKE08030 (ΔdusB2::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATAACAAAAATCAAATCCT,  downstream forward: _UP4_TAACAGCAGTGCTTGATTCA
BKK08030 (ΔdusB2::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATAACAAAAATCAAATCCT,  downstream forward: _UP4_TAACAGCAGTGCTTGATTCA
References
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B1C2BD8CEEABF9BBAE2646E8E24457A485097CFC

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Time of last update: 2025-01-27 00:56:34

Author of last update: Jstuelk