pnbA

pnbA
168

para-nitrobenzyl esterase

Locus
BSU_34390
Molecular weight
53.82 kDa
Isoelectric point
4.78
Protein length
489 aaSequenceBlast
Gene length
1470 bpSequenceBlast
Function
lipid degradation
Product
para-nitrobenzyl esterase
Essential
no
E.C.
3.1.1.3
Synonyms
pnbA, estB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2272 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,530,635  3,532,104
The protein
Protein family
type-B carboxylesterase/lipase family (single member, according to UniProt)
Structure
1C7J (PDB)\tPubMed
P37967 (AlphaFold)
Modification
phosphorylation on Ser-189 PubMed
Expression and Regulation
Operons
Genes
pnbA
Description
PubMed
Regulation
repressed during logarithmic growth (AbrB) PubMed
Regulatory mechanism
AbrB: repression, PubMed, in abrB regulon
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pnbA

2025-03-14 21:04:49

Bzhu

56

7a21ec2d4230db9280251b2ac8104c41c8474d94

412BAC029CDBBD64F40ED1442F0B7853C0F28BED

Genes
slrR-pnbA
Description
PubMed
Regulatory mechanism
Abh: activation, PubMed, in abh regulon
SinR: repression, PubMed, in sinR regulon
AbrB: repression, PubMed, in abrB regulon
Additional information
induction by sequestration of SinR by SinI, SlrA PubMed or by SlrR itself PubMed
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slrR->pnbA

2025-03-16 17:36:47

Jstuelk

119

3358c5e18cdab4c493893062172b97a17de9a83a

95EEFC91581CAC91E7EB53DE46FEEBE061AAC2B5

Biological materials
Mutant
GP951 (pnbA::cat), available in Jörg Stülke's lab
GP955 (''slrR-pnbA::cat''), available in Jörg Stülke's lab
BKE34390 (pnbA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTGTTCTCTCCCTTTT,  downstream forward: _UP4_TAAATATGGGGAAAACAGGA
BKK34390 (pnbA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTGTTCTCTCCCTTTT,  downstream forward: _UP4_TAAATATGGGGAAAACAGGA
References
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7FCB9037FDDC176FCF76088ADE7387A6A37068A9

Page visits: 4926

Time of last update: 2025-04-04 00:56:56

Author of last update: Jstuelk